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Table 4 Residual inhibitory power of IOS and the LF after enzymatic treatment

From: Strong cellulase inhibitors from the hydrothermal pretreatment of wheat straw

  

Residual inhibitory powera(%)

Enzyme

Enzyme concentration

IOSb

LFc

none

-

100

100

Tr Cel12A

3.5 μM

3.0 ± 0.8

45 ± 10

Tr Cel7B

3.5 μM

3.8 ± 0.3

16 ± 2

Ta Xyn10A

1.75 μM

9.9 ± 1.6

85 ± 4

Tr Cel5A

3.5 μM

13.1 ± 2.1

43 ± 1.4

Tr Cel7A

21 μM

15.6 ± 2.2

55 ± 9

Tr Cel6A

7.0 μM

24.9 ± 3.6

49 ± 8

N188 BG

1.75 μM

26.0 ± 2.4

49 ± 9

Celluclast/N188

1.75 FPU/ml

n.d.d

6.9 ± 0.6

4.55 CBU/ml

Thermomixe

2.8 mg/ml (1.8 FPU/ml)

n.d.d

3.1 ± 0.3

Tr AXE

0.1 μM

96.8 ± 0.1

n.d.d

Tr XG

0.1 μM

29.0 ± 9.4

n.d.d

Lichenase

0.1 μM

45.0 ± 4.2

n.d.d

  1. aInhibitory power was measured for Tr Cel7A on MUL substrate.
  2. bIOS at 100 μM were treated with the indicated enzyme at 35°C for 2 h.
  3. cThe LF in its original concentration (Table 2) was treated with the indicated enzyme at 35°C for 2 h.
  4. dNot determined.
  5. eThermomix contains Cel7A from Acremonium thermophilum, Cel6A from Chaetomium thermophilum, and EG, β-glucosidase, and xylanase from Thermoascus aurantiacus[55].