Differences in contact maps between native and thermostable mutant (A35V) GH12 endoglucanase proteins. Contacts present in the native but missing from the A35V mutant are in hollow squares. Similarly, contacts present in A35V but missing from native are in black squares. (a) Shows the hydrophobic interactions while (b) shows the hydrogen bonds at Θ = 346 K. Notice that the thermostable mutant in general has more contacts that are further off the slope = 1 diagonal leading to an increase in contact order (CO). This figure is reproduced from, published with permission from Taylor & Francis.