Table 1 Summary of RNA-Seq sequencing and data analysis results
Samples from R1 | R11 | R12 | R13 | R14 | R15 | R16 | R17 | R18 | --- | Total |
|---|---|---|---|---|---|---|---|---|---|---|
Time collected (h) | 3 | 5 | 7 | 10 | 13 | 16 | 20 | 28 | --- | Â |
Total number of reads | 32934519 | 29367899 | 15075431 | 30451579 | 37230096 | 26184184 | 36117022 | 32444284 | --- | 241287505 |
No. of reads mapped | 31098649 | 27046332 | 14016757 | 29094448 | 34907591 | 24836210 | 34255629 | 30754172 | --- | 227240126 |
No. of reads unambiguously mapped | 29844200 | 26282300 | 13592284 | 28326600 | 34233900 | 24012800 | 33747700 | 30065800 | --- | 221417584 |
No. of genes with detectable expression1 | 5070 | 5056 | 5032 | 5075 | 5084 | 5062 | 5087 | 5085 | --- | 5088 |
Range in expression levels (RPKM) | 7.4 × 10-3 | 1.1 × 10-2 | 5.7 × 10-3 | 2.3 × 10-2 | 1.6 × 10-3 | 1.5 × 10-3 | 2.3 × 10-2 | 2.3 × 10-2 | --- | 1.5 × 10-3 |
~ 2.0 × 104 | ~ 1.8 × 105 | ~ 8.6 × 104 | ~ 5.0 × 104 | ~ 9.2 × 104 | ~ 1.1 × 105 | ~ 2.2 × 105 | ~ 8.8 × 104 |  | ~ 2.2 × 105 | |
Samples from R2 | R21 | R22 | R23 | R24 | R25 | R26 | R27 | R28 | R23-22 | Total3 |
Time collected (h) | 10 | 12 | 14 | 17 | 20 | 23 | 28 | 36 | 14 | Â |
Total number of reads | 25350444 | 38025575 | 31352222 | 33877714 | 26099516 | 42485421 | 38176862 | 33624379 | 33916811 | 268992133 |
No. of reads mapped | 23995017 | 35773620 | 29542453 | 32664767 | 24890605 | 40877908 | 36397654 | 31652246 | 32399849 | 255794270 |
No. of reads unambiguously mapped | 22098900 | 31363000 | 27534800 | 31668100 | 24198000 | 39820500 | 35617800 | 30760200 | 31451300 | 243061300 |
No. of genes with detectable expression1 | 5059 | 5063 | 5058 | 5077 | 5084 | 5088 | 5088 | 5084 | 5081 | 5088 |
Range in expression levels (RPKM) | 2.3 × 10-2 | 6.2 × 10-3 | 2.4 × 10-2 | 1.6 × 10-3 | 2.8 × 10-2 | 4.7 × 10-2 | 2.6 × 10-2 | 3.6 × 10-2 | 2.9 × 10-2 | 1.6 × 10-3 |
|  | ~ 1.1 × 105 | ~ 1.0 × 105 | ~ 1.3 × 105 | ~ 1.5 × 105 | ~ 4.6 × 104 | ~ 6.7 × 104 | ~ 1.3 × 105 | ~ 1.2 × 105 | ~ 1.1 × 105 | ~ 1.5 × 105 |