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Figure 2 | Biotechnology for Biofuels

Figure 2

From: Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli

Figure 2

A schematic representation of the triple-plasmid expression systems utilized for 1-propanol production. All strains have (A) sbm-ygfD-ygfG cloned into pK184 under the control of p lac as well as, (B) sucCD cloned into pBBR1MCS-3 under the control of the arabinose inducible p araB . In addition to these, each strain has (C) pUC19 containing one of the seven listed alcohol dehydrogenases. The red star in the adhEMUT(EC) represents the E (glu)→K (lys) mutation at amino acid residue 568.

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