Figure 4
End point secretion profile of major end products from anaerobic fermentations of WT-adhE2(CA)3at five optical densities (OD600), profiled by 1D-1H-NMR. Major end products that are competitor metabolites to the production of 1-propanol are quantified by the left axis. Products detected along the desired metabolic pathway towards formation of 1-propanol are quantified by the right axis. Strain was cultivated anaerobically in reduced M9 minimal media with 20 g/L of glucose at 37°C for 72 h.