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Table 1 1-Propanol and other metabolite titers (mg/L) in reduced M9 minimal media using E. coli strain BW25141 transformed with appropriate plasmids

From: Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli

Strain

Carbon source

Metabolite titers (mg/L)

Succinate

Lactate

Acetate

Propionate

Ethanol

1-Propanol

Control

BW25141

Glucose

307 ± 36

128 ± 11

4436 ± 250

—

3021 ± 156

—

Experimental

WT-adhE2(CA)2

Glucose

264 ± 8

2601 ± 642

2961 ± 72

Trace

2640 ± 170

47 ± 2

WT-adhE2(CA)3

Glucose

231 ± 11

1877 ± 303

2653 ± 55

51 ± 14

3199 ± 283

103 ± 16

WT-adhE2(CA)3

Glucose and succinate

2200 ± 172

2293 ± 2970

3699 ± 352

123 ± 21

2774 ± 297

168 ± 39

WT-adhE2(CA)3-∆ygfD

Glucose

269 ± 94

3970 ± 1367

2527 ± 142

52 ± 8

1999 ± 104

37 ± 1

  1. Cultures were induced at an O.D600 of 15. Strains were cultivated anaerobically at 37°C for 72 h. Carbon sources: 20 g/L glucose and 4 g/L succinate where indicated. All experiments were performed in triplicate.