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Table 3 Secretion profile of the metabolites produced (mg/L) by various knock out strains with or without appropriate plasmids

From: Manipulating the sleeping beauty mutase operon for the production of 1-propanol in engineered Escherichia coli

Strain

Metabolite titers (mg/L)

Succinate

Lactate

Acetate

Propionate

Ethanol

1-Propanol

Controls

      

BW25141

307 ± 36

128 ± 11

4436 ± 250

—

3021 ± 156

—

WT-∆adhE

Trace

99 ± 17

4646 ± 705.2

—

1936 ± 741.9

—

WT-∆pta

776 ± 57

7259 ± 14

694 ± 196

—

3927 ± 691

—

WT-∆ldhA

187 ± 7

195 ± 14

3960 ± 151

—

6128 ± 80

—

Experimental

      

WT-adhE2(CA)3

231 ± 11

1877 ± 303

2653 ± 55

51 ± 14

2774 ± 297

103 ± 16

∆ldhA- adhE(EC)

206 ± 49

63 ± 3

4181 ± 550

—

6209 ± 183

42 ± 4

∆ldhA- adhE2(CA)

247 ± 64

77 ± 4

4210 ± 292

—

6713 ± 270

57 ± 1

∆ldhA- adhE1(CA)

256 ± 106

81 ± 10

3696 ± 652

—

5863 ± 9

45 ± 10

∆ldhA- adhEMUT(EC)

243 ± 8

79 ± 7

3814 ± 26

—

6021 ± 104

60 ± 9

∆ldhA- adhP(EC)

208 ± 115

190 ± 16

4488 ± 126

—

6124 ± 119

65 ± 2

∆ldhA-yqhD(EC)

145 ± 49

99 ± 16

4145 ± 14

—

5732 ± 77

38 ± 1

∆ldhA- bdhB(CA)

212 ± 50

89 ± 12

4351 ± 204

—

5652 ± 195

41 ± 4

  1. Cultures were suspended in reduced M9 minimal media and induced at an O.D600 of 15. Strains were cultivated anaerobically at 37°C for 72 h. Glucose (20 g/L) was used as the sole carbon source and all experiments were performed in triplicate.