Table 5 E. coli strains containing variants of the synthetic 1-propanol pathway used in this study
Strain | E. coli host | Plasmid 1 | Plasmid 2 | Plasmid 3 |
|---|---|---|---|---|
WT2 | BW25141 | pK-scpAKB | pB-sucCD | — |
WT-adhE2(CA)2 | BW25141 | pK-scpAKB | — | pU-adhE2(CA) |
WT-adhE2(CA)3 | BW25141 | pK-scpAKB | pB-sucCD | pU-adhE2(CA) |
WT-adhE2(CA)3-∆ygfD | BW25141 | pK-scpAB | pB-sucCD | pU-adhE2(CA) |
WT-adhE1(CA) | BW25141 | pK-scpAKB | pB-sucCD | pU-adhE1(CA) |
WT-adhEMUT(EC) | BW25141 | pK-scpAKB | pB-sucCD | pU-adhEMUT(EC) |
WT-adhP(EC) | BW25141 | pK-scpAKB | pB-sucCD | pU-adhP(EC) |
WT-yqhD(EC) | BW25141 | pK-scpAKB | pB-sucCD | pU-yqhD(EC) |
WT-bdhB(CA) | BW25141 | pK-scpAKB | pB-sucCD | pU-bdhB(CA) |
∆ldhA-adhE(EC) | WT-∆ldhA | pK-scpAKB | pB-sucCD | pU-adhE(EC) |
∆ldhA-adhE2(CA) | WT-∆ldhA | pK-scpAKB | pB-sucCD | pU-adhE2(CA) |
∆ldhA-adhE1(CA) | WT-∆ldhA | pK-scpAKB | pB-sucCD | pU-adhE1(CA) |
∆ldhA-adhEMUT (EC) | WT-∆ldhA | pK-scpAKB | pB-sucCD | pU-adhEMUT(EC) |
∆ldhA-adhP(EC) | WT-∆ldhA | pK-scpAKB | pB-sucCD | pU-adhP(EC) |
∆ldhA-yqhD(EC) | WT-∆ldhA | pK-scpAKB | pB-sucCD | pU-yqhD(EC) |
∆ldhA-bdhB(CA) | WT-∆ldhA | pK-scpAKB | pB-sucCD | pU-bdhB(CA) |