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Figure 4 | Biotechnology for Biofuels

Figure 4

From: Improvement of plant growth and seed yield in Jatropha curcas by a novel nitrogen-fixing root associated Enterobacter species

Figure 4

Effect of Enterobacte r R4-368 inoculation on seed production. Plants were planted in pots in sterilized soil (compost/sand mix at 1:1 ratio and in ɸ23 cm, 18 cm height pots; named as Trial I) or none-sterilized soil (nutrient poor clay soil in ɸ30 cm, 28 cm height pots; named as Trial II). Trial I and Trial II were maintained in different locations and started in different seasons. R4-368 cell suspension (1.2 OD600nm, 50 ml/pot) was applied to the plant root system at 21 days after seed germination. Fertiliser was applied regularly at about half of the recommended dose of approximately 50:30:30 g/plant/year. (A) Plant height (B) leaf canopy. Seed set numbers per plant (C) (n = 8 in Trial I and n = 12 in Trial II) were measured at 480 and 520 DAI in Trail I and Trail II respectively and single seed weight (D) was calculated based the average of 180 randomly selected seeds per treatment except the non-treated for Trial I where only 117 seeds were measured. Error bars indicate standard error. * and ** indicate statistically significant between the treated and non-treated populations (*P < 0.05 for seed set per plant and **P < 0.01 for single seed weight) by Student’s t-test.

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