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Table 2 Effect of nitrogen fixation genes on growth promotion activity of R4-368 under N-limiting conditions

From: Improvement of plant growth and seed yield in Jatropha curcas by a novel nitrogen-fixing root associated Enterobacter species

Treatments1 Plant height (cm)* Number of leaves* Shoot biomass (g)* Root biomass (g)* Leaf N content (%)# N-fixing population (log CFU/g)§ In planta AR activity (nmol C2H4/h/g)§
Wild type 34.0 ± 5.8 10.0 ± 2.4 7.1 ± 0.7 1.4 ± 0.1 1.97 ± 0.32 7.47 ± 0.05 49.94 ± 3.18
ΔnifH 25.3 ± 4.5b 8.1 ± 1.2 4.1 ± 1.3b 0.7 ± 0.1 1.06 ± 0.01 5.86 ± 0.10b 4.52 ± 2.46b
ΔnifD 23.1 ± 4.5b 7.1 ± 1.2b 4.4 ± 0.4b 0.6 ± 0.1b 0.88 ± 0.02b 5.78 ± 0.21b 10.47 ± 5.20b
ΔnifK 22.6 ± 3.9b 7.3 ± 1.3b 4.4 ± 0.5b 0.8 ± 0.1 1.26 ± 0.03 5.69 ± 0.14b 8.12 ± 2.55b
Mock 17.9 ± 1.3 6.1 ± 1.2b 2.6 ± 0.5 0.6 ± 0.0 b 0.90 ± 0.03b 5.79 ± 0.15b 8.08 ± 2.23b
  1. 1Bacterial inoculants (50 ml/pot) were applied near the root regions after transplanting while the control plant was applied with sterile distilled water. Plants were grown in the open air in a greenhouse. Plants were watered as needed with a nutrient solution containing 5 μM CaCl2, 1.25 μM MgSO4, 5 μM KCl, 1 μM KH2PO4, 0.162 μM FeSO4, 2.91 nM H3BO3, 1.14 nM MnSO4, 0.76 nM ZnSO4, 0.13 nM NaMoO4, 0.14 nM NiCl2, 0.013 nM CoCl2, and 0.19 nM CuSO4.
  2. bThe treatments are not significantly different from each other at 5% threshold.
  3. *, n = 7; #, n = 4; and §, 45 DAI.
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