1Wt and nif mutants were labelled with pMC1-EPS-RtGFP and inoculated to root zones of 12 day-old seedlings derived from surface-sterilized. Plants were maintained in sterile conditions in sand-nutrient mix in Phytatrays.
2Number of CFU (per g dry biomass) recovered from surface-sterilized root tissues of Jatropha curcas cv. MD-44. Bacteria were recovered on 2xYT medium supplemented with chloramphenical (100 μg/ml).
aThe treatments are not significantly different from each other at 5% threshold.