Improvement of plant growth and seed yield in Jatropha curcas by a novel nitrogen-fixing root associated Enterobacter species

Table 3 Bacterial density at 45 DAI under gnotobiotic conditions

Treatments¹	Endophytic population (log cfu/g)²
	Root	Stem	Leaves
Wild type	7.83 ± 0.10^a	7.13 ± 0.05^a	0
nifH Δ	7.62 ± 0.16^a	7.02 ± 0.19^a	0
nifD Δ	7.67 ± 0.20^a	6.91 ± 0.32^a	0
nifK Δ	7.65 ± 0.14^a	6.98 ± 0.32^a	0
Mock	0	0	0

¹Wt and nif mutants were labelled with pMC1-EPS-RtGFP and inoculated to root zones of 12 day-old seedlings derived from surface-sterilized. Plants were maintained in sterile conditions in sand-nutrient mix in Phytatrays.
²Number of CFU (per g dry biomass) recovered from surface-sterilized root tissues of Jatropha curcas cv. MD-44. Bacteria were recovered on 2xYT medium supplemented with chloramphenical (100 μg/ml).
^aThe treatments are not significantly different from each other at 5% threshold.

ISSN: 2731-3654