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Table 5 Central composite design (CCD) matrix of three independent variables for H 2 production in actual values with experimental results of WS by employing Rhodobacter capsulatus -PK (SS-8) in the presence of tungsten lamp with light intensity of 120-150 W/m 2

From: Photo-biohydrogen production potential of Rhodobacter capsulatus- PK from wheat straw

Run No

Variables

Response

Yield

 

X1

X2

X3

Y hydrogen production (ml)

Mole of H2/mole of substrate

1

-1.000

-1.000

-1.000

528

0.98

2

-1.682

0.000

0.000

587

1.09

3

1.000

-1.000

-1.000

597

1.11

4

0.000

0.000

0.000

709

1.32

5

1.682

0.000

0.000

603

1.12

6

0.000

0.000

0.000

709

1.32

7

0.000

0.000

0.000

712

1.32

8

0.000

0.000

0.000

711

1.32

9

1.000

-1.000

1.000

561

1.04

10

0.000

1.682

0.000

598

1.11

11

0.000

0.000

1.682

551

1.02

12

0.000

0.000

-1.682

559

1.04

13

0.000

0.000

0.000

709

1.32

14

0.000

-1.682

0.000

554

1.03

15

0.000

0.000

0.000

709

1.32

16

1.000

1.000

1.000

542

1.01

17

-1.000

1.000

-1.000

568

1.06

18

-1.000

1.000

1.000

572

1.06

19

1.000

1.000

-1.000

540

1.00

20

-1.000

-1.000

1.000

527

0.98