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Table 5 Central composite design (CCD) matrix of three independent variables for H 2 production in actual values with experimental results of WS by employing Rhodobacter capsulatus -PK (SS-8) in the presence of tungsten lamp with light intensity of 120-150 W/m 2

From: Photo-biohydrogen production potential of Rhodobacter capsulatus- PK from wheat straw

Run No Variables Response Yield
  X1 X2 X3 Y hydrogen production (ml) Mole of H2/mole of substrate
1 -1.000 -1.000 -1.000 528 0.98
2 -1.682 0.000 0.000 587 1.09
3 1.000 -1.000 -1.000 597 1.11
4 0.000 0.000 0.000 709 1.32
5 1.682 0.000 0.000 603 1.12
6 0.000 0.000 0.000 709 1.32
7 0.000 0.000 0.000 712 1.32
8 0.000 0.000 0.000 711 1.32
9 1.000 -1.000 1.000 561 1.04
10 0.000 1.682 0.000 598 1.11
11 0.000 0.000 1.682 551 1.02
12 0.000 0.000 -1.682 559 1.04
13 0.000 0.000 0.000 709 1.32
14 0.000 -1.682 0.000 554 1.03
15 0.000 0.000 0.000 709 1.32
16 1.000 1.000 1.000 542 1.01
17 -1.000 1.000 -1.000 568 1.06
18 -1.000 1.000 1.000 572 1.06
19 1.000 1.000 -1.000 540 1.00
20 -1.000 -1.000 1.000 527 0.98