Lignin quantification from the leaf and stem of RG and wild type sorghum. (A, B, C, D) Maule’s staining for lignin in cross sections of sorghum stem (A, C) and leaf tissue (B, D). (E) Total insoluble lignin, (F) total soluble lignin; Each bar comprises the mean of four biological and four technical replicates. Error bars indicate the standard error from the mean. Significance (P < 0.05) is indicated by a star (★). Scale bar = 1 mm.