Xylo- and cello-oligosaccharide oxidation by gluco-oligosaccharide oxidase from Sarocladium strictumand variants with reduced substrate inhibition

Vuong, Thu V; Vesterinen, Arja-Helena; Foumani, Maryam; Juvonen, Minna; Seppälä, Jukka; Tenkanen, Maija; Master, Emma R

doi:10.1186/1754-6834-6-148

Biotechnology for Biofuels and Bioproducts

Table 2 Kinetic parameters of GOOX, GOOX-VN (A38V-S388N) and GOOX-V (A38V) on cello-oligosaccharides and xylo-oligosaccharides

From: Xylo- and cello-oligosaccharide oxidation by gluco-oligosaccharide oxidase from Sarocladium strictumand variants with reduced substrate inhibition

	GOOX			GOOX-VN (A38V-S388N)			GOOX-V (A38V)
	k_cat(min^-1)	K_m(mM)	k_cat/K_m(mM^-1min^-1)	k_cat(min^-1)	K_m(mM)	k_cat/K_m(mM^-1min^-1)	k_cat(min^-1)	K_m(mM)	k_cat/K_m(mM^-1min^-1)
Glucose	378 ± 5^*	15 ± 1	25 ± 2	361 ± 5	15 ± 1	24 ± 2	625 ± 12	12 ± 1	50 ± 5
Cellobiose	420 ± 20	0.04 ± 0.01	9,400 ± 2,000	372 ± 14	0.05 ± 0.01	7,000 ± 1,000	719 ± 8	0.039 ± 0.002	18,400 ± 900
Cellotriose	412 ± 16	0.05 ± 0.01	8,600 ± 1,000	436 ± 14	0.08 ± 0.01	5,600 ± 600	820 ± 50	0.05 ± 0.01	15,000 ± 3,000
Cellotetraose	595 ± 16	0.07 ± 0.01	9,000 ± 800	470 ± 12	0.09 ± 0.01	5,200 ± 500	770 ± 20	0.06 ± 0.01	14,000 ± 2,000
Cellopentaose	498 ± 19	0.06 ± 0.01	8,700 ± 1,100	453 ± 14	0.09 ± 0.01	4,700 ± 400	760 ± 30	0.057 ± 0.007	13,000 ± 2,000
Cellohexaose	430 ± 30	0.08 ± 0.02	5,500 ± 1,200	393 ± 15	0.10 ± 0.01	4,000 ± 400	790 ± 30	0.08 ± 0.01	9,000 ± 1,000
Xylose	444 ± 12	129 ± 8	3.4 ± 0.2	330 ± 8	118 ± 7	2.8 ± 0.2	741 ± 15	118 ± 7	6.3 ± 0.4
Xylobiose	522 ± 8	0.057 ± 0.003	9,100 ± 500	449 ± 5	0.099 ± 0.005	4,500 ± 200	890 ± 20	0.05 ± 0.01	18,000 ± 2,000
Xylotriose	620 ± 14	0.08 ± 0.01	8,200 ± 700	524 ± 10	0.10 ± 0.01	5,100 ± 400	870 ± 30	0.08 ± 0.01	11,000 ± 1,000
Xylotetraose	583 ± 14	0.07 ± 0.01	8,200 ± 800	488 ± 7	0.11 ± 0.01	4,400 ± 300	960 ± 30	0.067 ± 0.007	14,000 ± 1,000
Xylopentaose	623 ± 18	0.05 ± 0.01	11,800 ± 1,400	529 ± 7	0.104 ± 0.005	5,100 ± 200	910 ± 30	0.05 ± 0.01	17,000 ± 2,000
Xylohexaose	516 ± 18	0.06 ± 0.01	8,100 ± 1,200	465 ± 7	0.10 ± 0.01	4,400 ± 300	910 ± 20	0.050 ± 0.004	18,000 ± 2,000
A³X	530 ± 13	0.059 ± 0.005	8,900 ± 700	ND	ND	ND	740 ± 14	0.052 ± 0.004	14,000 ± 1,000
A^d2+3XX	540 ± 11	0.078 ± 0.004	6,900 ± 400	ND	ND	ND	810 ± 20	0.09 ± 0.01	9,000 ± 700
U^4m2XX	659 ± 9	0.30 ± 0.01	2,190 ± 60	ND	ND	ND	ND	ND	ND

*Data are mean values ± standard errors; 16 nM of enzyme was used in each reaction.
ND Not determined.

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ISSN: 2731-3654

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