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Table 2 Kinetic parameters of GOOX, GOOX-VN (A38V-S388N) and GOOX-V (A38V) on cello-oligosaccharides and xylo-oligosaccharides

From: Xylo- and cello-oligosaccharide oxidation by gluco-oligosaccharide oxidase from Sarocladium strictumand variants with reduced substrate inhibition

  GOOX GOOX-VN (A38V-S388N) GOOX-V (A38V)
  kcat(min-1) Km(mM) kcat/Km(mM-1min-1) kcat(min-1) Km(mM) kcat/Km(mM-1min-1) kcat(min-1) Km(mM) kcat/Km(mM-1min-1)
Glucose 378 ± 5* 15 ± 1 25 ± 2 361 ± 5 15 ± 1 24 ± 2 625 ± 12 12 ± 1 50 ± 5
Cellobiose 420 ± 20 0.04 ± 0.01 9,400 ± 2,000 372 ± 14 0.05 ± 0.01 7,000 ± 1,000 719 ± 8 0.039 ± 0.002 18,400 ± 900
Cellotriose 412 ± 16 0.05 ± 0.01 8,600 ± 1,000 436 ± 14 0.08 ± 0.01 5,600 ± 600 820 ± 50 0.05 ± 0.01 15,000 ± 3,000
Cellotetraose 595 ± 16 0.07 ± 0.01 9,000 ± 800 470 ± 12 0.09 ± 0.01 5,200 ± 500 770 ± 20 0.06 ± 0.01 14,000 ± 2,000
Cellopentaose 498 ± 19 0.06 ± 0.01 8,700 ± 1,100 453 ± 14 0.09 ± 0.01 4,700 ± 400 760 ± 30 0.057 ± 0.007 13,000 ± 2,000
Cellohexaose 430 ± 30 0.08 ± 0.02 5,500 ± 1,200 393 ± 15 0.10 ± 0.01 4,000 ± 400 790 ± 30 0.08 ± 0.01 9,000 ± 1,000
Xylose 444 ± 12 129 ± 8 3.4 ± 0.2 330 ± 8 118 ± 7 2.8 ± 0.2 741 ± 15 118 ± 7 6.3 ± 0.4
Xylobiose 522 ± 8 0.057 ± 0.003 9,100 ± 500 449 ± 5 0.099 ± 0.005 4,500 ± 200 890 ± 20 0.05 ± 0.01 18,000 ± 2,000
Xylotriose 620 ± 14 0.08 ± 0.01 8,200 ± 700 524 ± 10 0.10 ± 0.01 5,100 ± 400 870 ± 30 0.08 ± 0.01 11,000 ± 1,000
Xylotetraose 583 ± 14 0.07 ± 0.01 8,200 ± 800 488 ± 7 0.11 ± 0.01 4,400 ± 300 960 ± 30 0.067 ± 0.007 14,000 ± 1,000
Xylopentaose 623 ± 18 0.05 ± 0.01 11,800 ± 1,400 529 ± 7 0.104 ± 0.005 5,100 ± 200 910 ± 30 0.05 ± 0.01 17,000 ± 2,000
Xylohexaose 516 ± 18 0.06 ± 0.01 8,100 ± 1,200 465 ± 7 0.10 ± 0.01 4,400 ± 300 910 ± 20 0.050 ± 0.004 18,000 ± 2,000
A3X 530 ± 13 0.059 ± 0.005 8,900 ± 700 ND ND ND 740 ± 14 0.052 ± 0.004 14,000 ± 1,000
Ad2+3XX 540 ± 11 0.078 ± 0.004 6,900 ± 400 ND ND ND 810 ± 20 0.09 ± 0.01 9,000 ± 700
U4m2XX 659 ± 9 0.30 ± 0.01 2,190 ± 60 ND ND ND ND ND ND
  1. *Data are mean values ± standard errors; 16 nM of enzyme was used in each reaction.
  2. ND Not determined.