Skip to main content

Table 4 Substrate inhibition of GOOX and mutant variants

From: Xylo- and cello-oligosaccharide oxidation by gluco-oligosaccharide oxidase from Sarocladium strictumand variants with reduced substrate inhibition

Substrate GOOX GOOX-VN GOOX-V Y300A W351A
Vi/Vmax(%) Ki(mM)* n H** Vi/Vmax(%) Ki(mM) n H Vi/Vmax(%) Ki(mM) n H
Glucose NI NI NI NI NI
Cellobiose 32 2.51 ± 0.18 2.2 38 3.2 ± 0.2 1.8 35 2.7 ± 0.2 1.3 NI NI
Cellotriose 34 1.33 ± 0.13 1.4 42 1.9 ± 0.2 1.3 37 1.56 ± 0.13 1.9 NI NI
Cellotetraose 34 1.49 ± 0.10 1.8 42 2.20 ± 0.16 1.5 36 1.61 ± 0.12 1.6 NI NI
Cellopentaose 35 1.53 ± 0.13 1.4 53 1.66 ± 0.18 1.3 43 1.36 ± 0.11 1.4 NI NI
Cellohexaose 44 0.62 ± 0.09 1.3 54 0.81 ± 0.10 1.1 44 0.83 ± 0.07 1.2 NI NI
Xylose ID ID ID NI NI
Xylobiose 51 3.1 ± 0.2 1.2 70 5.0 ± 0.6 1.1 57 3.0 ± 0.2 1.5 NI NI
Xylotriose 45 2.99 ± 0.19 1.3 75 3.6 ± 0.4 1.2 49 3.0 ± 0.3 1.5 NI NI
Xylotetraose 57 1.94 ± 0.16 1.3 NI 62 1.82 ± 0.15 1.3 NI NI
Xylopentaose 53 2.0 ± 0.3 1.4 NI 56 2.0 ± 0.4 1.4 ND NI
Xylohexaose 61 1.6 ± 0.2 1.5 NI 54 2.2 ± 0.3 1.3 NI NI
  1. *Substrate inhibition kinetics derived from eq. 2. **Hill coefficient.
  2. -Values could not be calculated due to no detectable activity.
  3. NINo inhibition detected at tested substrate concentrations; IDInsufficient data to calculate inhibition kinetics; however inhibition was observed at xylose concentrations greater than 800 mM; NDNot determined.