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Table 1 Functional classification and quantitation of the same proteins identified in different treatments

From: Secretome diversity and quantitative analysis of cellulolytic Aspergillus fumigatusZ5 in the presence of different carbon sources

Accession no. Name of proteins LIBER number peptides num unique peps tot indep spectra percent share of spectrum id's iTRAQ Ratio 116/119 117/119 Family Signal P
116 117 119
Cellulase
gi|66844580 glycosyl hydrolase family 43 protein 4 1 7 0.21 0.16±0.03 0.11±0.01 0.03±0.01 5.33 3.67 GH 43 (PF04616) Y
gi|66845136 beta-1,6-glucanase Neg1 2 1 2 0.13 0.17±0.02 0.47±0.02 0.10±0 1.70 4.70 GH 30 (PF02055) Y
gi|66845978 beta-1,3-endoglucanase EglC 25 2 29 1.89 0.04±0.01 0.63±0.03 0.11±0.01 0.36 5.73 GH 17 (PF00332) Y
gi|66846140 cellobiohydrolase 10 1 12 0.76 0.19±0.02 0.10±0.01 0.09±0.01 2.11 1.11 GH 6 (PF01341) Y
gi|66846336 endo-1,4-beta-glucanase 10 3 10 0.64 0.24±0.01 0.09±0.02 0.13±0.02 1.85 0.69 GH 61 (PF03443) Y
gi|66846525 endoglucanase 6 2 6 0.39 0.27±0.03 0.14±0.01 0.19±0.04 1.42 0.74 GH 12 (PF01670) Y
gi|66846526 beta-D-glucoside glucohydrolase 13 2 15 0.97 0.28±0.01 0.16±0.01 0.11±0.01 2.55 1.45 GH 3 (PF00933) Y
gi|66846837 glucan 1,4-alpha-glucosidase 3 2 1 0.13 0.31±0.04 0.18±0.09 0.05±0.01 6.20 3.60 GH 15 (PF00723) Y
gi|66846860 extracellular glycosyl hydrolase 1 1 1 0.09 0.17±0.03 0.15±0.01 0.07±0.01 2.43 2.14 GH 62 (PF03664) Y
gi|66848676 endoglucanase 2 1 2 0.13 0.33±0.07 0.10±0 0.03±0 11.00 3.33 GH 5 (PF00150) Y
gi|66849674 endoglucanase 58 15 109 6.98 0.31±0.01 0.12±0 0.04±0 7.75 3.00 GH 61 (PM03443) Y
gi|66850620 extracellular cell wall glucanase 1 1 1 0.07 0.2±0.04 0.55±0.07 0.06±0 3.33 9.17 GH 16 (PF00722) Y
gi|66850743 beta-glucosidase 1 2 1 0.15 0.42±0 0.26±0.02 0.06±0 7.00 4.33 GH 3 (PF01915) Y
gi|74670557 1,4-beta-D-glucan-cellobiohydrolyase 21 12 100 7.45 0.28±0.01 0.11±0.01 0.06±0.01 4.67 1.83 GH 7 (PF00840) Y
gi|74670999 cellobiohydrolase 17 12 35 3.22 0.30±0.01 0.09±0.01 0.06±0.01 5.00 1.50 GH 7 (PF00840) Y
Hemicellulase
gi|66845449 alpha-galactosidase 2 2 3 0.12 0.25±0.01 0.13±0.11 0.11±0.08 2.27 1.18 Melibiase (PF02065) N
gi|66845983 endo-1,4-beta-xylanase (XlnA) 31 9 49 3.16 0.34±0.01 0.07±0 0.02±0.01 17.00 3.50 GH 11 (PF00457) Y
gi|66846833 arabinosidase 1 2 2 0.08 0.44±0.09 0.10±0 0.03±0 14.67 3.33 GH 43 (PF04616) N
gi|66846861 xylosidase 1 2 4 0.20 0.72±0 0.21±0 0.01±0 72.00 21.00 GH 43 (PF04616) Y
gi|66848870 endo-1,4-beta-xylanase 21 7 35 2.27 0.35±0.01 0.08±0.01 0.05±0.01 7.00 1.60 GH 10 ( PF00331 ) Y
gi|66850460 mannosidase MsdS 1 2 6 0.09 0.44±0.07 0.06±0.01 0.13±0.02 3.38 0.46 GH 47 ( PF01532) Y
gi|66851740 endo-1,4-beta-xylanase 2 2 3 0.12 0.35±0.02 0.13±0.05 0.11±0.08 3.18 1.18 GH 10 ( PF00331) Y
gi|70981394 extracellular arabinanase 4 3 4 0.26 0.27±0.03 0.24±0.01 0.11±0.06 2.45 2.18 GH 43 (PF04616) Y
gi|70994060 extracellular endo-1,4-beta-xylanase 17 10 26 1.62 0.31±0.01 0.08±0 0.06±0.01 5.17 1.33 GH 10 (PF00331) Y
gi|74668470 endo-1,4-beta-xylanase 5 3 5 0.23 0.28±0.05 0.24±0.01 0.13±0.06 2.15 1.85 GH 11 (PF00457) Y
Chitinase
gi|129556953 class V chitinase 1 1 1 0.09 0.27±0.03 0.10±0.01 0.35±0.07 0.77 0.29 GH18 (PF00704) Y
gi|129558344 class V chitinase 6 8 14 0.86 0.24±0.01 0.26±0.01 0.1±0.02 2.40 2.60 GH 18 (PF00704) Y
gi|66846625 class III chitinase 5 5 5 0.30 0.19±0.04 0.17±0.06 0.15±0.06 1.27 1.13 GH 18 (PF00704) Y
gi|70983075 class V chitinase ChiB1 72 23 159 10.35 0.27±0.01 0.09±0 0.11±0.01 2.45 0.82 GH 18 (PF00704) Y
Phosphatase, lipase, pectinase, lignin degrading proteins, esterase and protease
gi|129557040 Ser/Thr protein phosphatase family protein 1 2 2 0.09 0.49±0.08 0.06±0.01 0.15±0.02 3.27 0.40 Metallophos (PF00149) N
gi|129558573 esterase 1 2 3 0.18 0.07±0 0.26±0.02 0.55±0.05 0.13 0.47 - Y
gi|66845766 extracellular lipase 37 7 64 4.12 0.07±0.01 0.34±0.01 0.35±0.02 0.20 0.97 Lipase 3 (PF01764) Y
gi|66846844 pectate lyase 1 1 1 0.07 0.73±0.04 0.15±0.01 0.25±0.04 2.92 0.60 Pec_lyase_C (PF00544) Y
gi|66849090 metalloprotease 10 4 13 0.84 0.38±0.07 0.1±0.03 0.07±0.04 5.43 1.43 Peptidase_M35 (MF02102) Y
gi|66853735 cellobiose dehydrogenase 10 3 12 0.79 0.16±0.02 0.41±0.03 0.19±0.02 0.84 2.16 GMC_oxred_N (PF00732) Y
gi|70986104 CAT1 mycelial catalase Cat1 8 5 10 0.60 0.21±0.02 0.09±0.01 0.25±0.02 0.84 0.36 Catalase (PF00199) N
gi|70997966 Cu, Zn superoxide dismutase 4 1 4 0.26 0.18±0.03 0.19±0.01 0.18±0.01 1.00 1.06 Sod_Cu (PF00080) N
Permease, reductase, helicase and Transporter
gi|66844481 high affinity methionine permease 2 1 2 0.13 0.28±0.01 0.19±0.02 0.17±0.02 1.65 1.12 AA_permease2 ( PF13520) N
gi|66848496 thioredoxin reductase GliT 2 4 4 0.23 0.14±0.08 0.37±0.06 0.29±0.01 0.48 1.28 Pyr_redox_2 (PF07992) N
gi|66848977 telomere-associated RecQ helicase 31 3 52 3.16 0.01±0 0.47±0 0.46±0.01 0.02 1.02 DUF3505 (PF12013) N
gi|70982865 MFS transporter 8 3 47 2.40 0.26±0 0.29±0.01 0.15±0 1.73 1.93 MFS_1 (PF07690) N
Other proteins
gi|129555482 cytochrome P450 5 2 5 0.29 0.11±0.03 0.27±0.03 0.31±0.10 0.35 0.87 p450 (PF00067) N
gi|129555486 secreted antimicrobial peptide 3 2 3 0.16 0.10±0.04 0.51±0.09 0.20±0.01 0.50 2.55 MiAMP1 (PF09117) Y
gi|146324637 hybrid PKS-NRPS enzyme 2 2 3 0.13 0.18±0.02 0.16±0.06 0.32±0.02 0.56 0.50 Acyl_transf_1 (PF00698) N
gi|66847207 HECT domain protein 5 3 8 0.51 0.29±0.03 0.12±0.01 0.15±0.02 1.93 0.80 HECT (PF00632) N
gi|66847547 C6 finger domain protein 2 1 2 0.13 0.02±0 0.51±0 0.39±0.02 0.05 1.31 Zn_clus (PF00172) N
gi|66851935 aldose 1-epimerase 2 2 13 0.11 0.26±0.04 0.16±0.06 0.32±0.07 0.81 0.50 Aldose-epim (PF01263) N
gi|66852539 glutaminase GtaA 2 2 3 0.18 0.40±0.01 0.03±0 0.07±0 5.71 0.43 DUF1793 (PF08760) Y
gi|66853400 translation initiation factor eIF-2B 49 1 64 3.95 0.30±0 0.27±0 0.09±0 3.33 3.00 W2 (PF02020) N
  1. The peptides from RS, Av and Gl were labeled with the iTRAQ tags 116, 117 and 119, respectively.