Figure 4

LS/Cel dependence of size and zeta potential for the LCCs in 50 mM buffer at pH 4.8 and 25°C. Various molar ratios of LS to Cel were realized by adding LS to 100 μL of Cel solution (1.5 mg/mL). The background image shows the mixtures with various molar ratios of LS to Cel at equilibrium. Cel, cellulase; LCC, lignosulfonate-cellulase complex; LS, lignosulfonate.