Reciprocal hemizygosity analysis for GUT1 . (A) Genetic background of the four diploid hybrids constructed by mating wild-type and gut1 deleted CBS 6412-13A and CEN.PK113-1A, respectively. All strains only differ genetically by the alleles of GUT1, that is, they carry either both alleles (GUT1
), one allele (GUT1
), or no alleles. (B) Growth of the hybrid strains in synthetic glycerol medium: hybrid I (red), hybrid II (blue), hybrid III (green), and hybrid IV (purple). Data from one representative experiment out of three independent biological replicates are shown.