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Table 2 S . cerevisiae strains used in this study

From: Re-evaluation of glycerol utilization in Saccharomyces cerevisiae: characterization of an isolate that grows on glycerol without supporting supplements

Strain

Genotype, description

Reference

S288c

MAT a (prototrophic)

Mortimer and Johnston [46]

W303-1A

MAT a (prototrophic)

Thomas and Rothstein [47]

CEN.PK113-7D

MAT a (prototrophic)

van Dijken et al. [48]

CEN.PK113-1A

MAT α (prototrophic)

P Kötter (Euroscarf)

CBS 6412

Wild-type, diploid

CBS strain collection

CBS 6412-13A

MAT a, haploid segregant of CBS 6412

This study

CBS 6412-13A gut1∆

MAT a gut1::loxP-kanMX-loxP

This study

CBS 6412-13A gut2∆

MAT a gut2::loxP-ble-loxP

This study

CBS 6412-13A stl1∆

MAT a stl1::loxP-ble-loxP

This study

CEN.PK113-1A gut1∆

MAT α gut1::loxP-kanMX-loxP

This study

Hybrid I

MAT a/α; cross of CBS 6412-13A and CEN.PK113-1A

This study

Hybrid II

MAT a/α; cross of CBS 6412-13A and CEN.PK113-1A gut1∆

This study

Hybrid III

MAT a/α; cross of CBS 6412-13A gut1∆ and CEN.PK113-1A

This study

Hybrid IV

MAT a/α; cross of CBS 6412-13A gut1∆ and CEN.PK113-1A gut1∆

This study

  1. The 52 S. cerevisiae strains used in the initial screening for glycerol growth are summarized in Additional file 1. Euroscarf, European Saccharomyces cerevisiae archive for functional analysis.