Re-evaluation of glycerol utilization in Saccharomyces cerevisiae: characterization of an isolate that grows on glycerol without supporting supplements

Table 4 Allele-specific primers used for the differentiation between the STL1 , GUT1, and GUT2 alleles from CEN.PK113-1A and CBS 6412-13A, respectively

Gene	Primer	Sequence (5′-3′)
STL1	Primers for specific amplification of STL1_CEN.PK113-1A	Forward: GGTTGTTTCGCAGGTTCTCTT
	Primers for specific amplification of STL1_CEN.PK113-1A	Reverse: ACTTCCTCATCATTTGGATCT
	Primers for specific amplification of STL1_{CBS 6412-13A}	Forward: GGTTGTTTCGCAGGTTCTCTA
	Primers for specific amplification of STL1_{CBS 6412-13A}	Reverse: ACTTCCTCATCATTTGGATCC
GUT1	Primers for specific amplification of GUT1_CEN.PK113-1A	Forward: GCAAACATGAGAGAAACCACA
	Primers for specific amplification of GUT1_CEN.PK113-1A	Reverse: AATTTCGGGCATGTGAATCAG
	Primers for specific amplification of GUT1_{CBS 6412-13A}	Forward: GCAAACATGAGAGAAACTACG
	Primers for specific amplification of GUT1_{CBS 6412-13A}	Reverse: AATTTCGGGCATGTGAATCAA
GUT2	Primers for specific amplification of GUT2_CEN.PK113-1A	Forward: CGCCACTTTAGCCATTACC
	Primers for specific amplification of GUT2_CEN.PK113-1A	Reverse: GTGTGACAAGGTTTCAGGTTG
	Primers for specific amplification of GUT2_{CBS 6412-13A}	Forward: CGCCACTTTAGCCATCACG
	Primers for specific amplification of GUT2_{CBS 6412-13A}	Reverse: GTGTGACAAGGTTTCAGGTTA

ISSN: 2731-3654