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Table 2 Comparison of several ethanol productions using Tween as an additive found in the literature and in the current study

From: Tween 40 pretreatment of unwashed water-insoluble solids of reed straw and corn stover pretreated with liquid hot water to obtain high concentrations of bioethanol

Raw material Pretreated method Tween additives Adding method of Tween Fermentation method Particle size of raw material Washed or unwashed with water Prehydrolysis time (hours) + fermentation time (hours) Enzyme loadings Ethanol concentration(g/L) Ethanol yield (%) Reference
Reed straw LHW Tween 40 Prior to fermentation Fed-batch S-SSF 3 to 5 cm Unwashed 18 + 72 25 FPU cellulase/g oven-dried WIS 56.28 69.1 This study
Corn stover LHW Tween 40 Prior to fermentation Fed-batch S-SSF 4 to 7 cm Unwashed 18 + 72 25 FPU cellulase/g oven-dried WIS 52.26 71.1 This study
Reed straw LHW - - Fed-batch S-SSF 20 to 80 mesh Washed 18 + 72 40 FPU cellulase/g oven-dried WIS 39.40 75.1 [27]
Corn stover LHW - - Fed-batch S-SSF 20 to 80 mesh Washed 18 + 72 40 FPU cellulase/g oven-dried WIS 39.40 74.4 In press
Wheat straw Sulfuric acid Tween 20 In the fermentation stage SSF 40 mesh Washed 72 20 FPU cellulase and 40 CBU β-glucosidase/g glucan 12.4 73.9 [24]
Spruce Steam pretreatment Tween 20 In the fermentation stage SSF 2 to 10 mm Washed 48 44 FPU/g cellulose 20 to 25 92 [15]
Sugarcane bagasse Dilute ammonia Tween 80 In the pretreatment stage SSF 0.05 to 1.5 cm Washed 72 30 FPU Spezyme CP and 30 CBU Novozyme 188/g glucan 18 g/100 g of dry biomass 69 [23]
Sugarcane bagasse Dilute ammonia Tween 20 In the pretreatment stage SSF 0.05 to 1.5 cm Washed 72 30 FPU Spezyme CP and 30 CBU Novozyme 188/g glucan 15 g/100 g of dry biomass 59 [23]
  1. CBU, cellobiase unit; LHW, liquid hot water; SSF, simultaneous saccharification and fermentation; S-SSF, semi-simultaneous saccharification and fermentation; WIS, water-insoluble solids.