Raw material | Pretreated method | Tween additives | Adding method of Tween | Fermentation method | Particle size of raw material | Washed or unwashed with water | Prehydrolysis time (hours) + fermentation time (hours) | Enzyme loadings | Ethanol concentration(g/L) | Ethanol yield (%) | Reference |
---|---|---|---|---|---|---|---|---|---|---|---|
Reed straw | LHW | Tween 40 | Prior to fermentation | Fed-batch S-SSF | 3 to 5 cm | Unwashed | 18 + 72 | 25 FPU cellulase/g oven-dried WIS | 56.28 | 69.1 | This study |
Corn stover | LHW | Tween 40 | Prior to fermentation | Fed-batch S-SSF | 4 to 7 cm | Unwashed | 18 + 72 | 25 FPU cellulase/g oven-dried WIS | 52.26 | 71.1 | This study |
Reed straw | LHW | - | - | Fed-batch S-SSF | 20 to 80 mesh | Washed | 18 + 72 | 40 FPU cellulase/g oven-dried WIS | 39.40 | 75.1 | [27] |
Corn stover | LHW | - | - | Fed-batch S-SSF | 20 to 80 mesh | Washed | 18 + 72 | 40 FPU cellulase/g oven-dried WIS | 39.40 | 74.4 | In press |
Wheat straw | Sulfuric acid | Tween 20 | In the fermentation stage | SSF | 40 mesh | Washed | 72 | 20 FPU cellulase and 40 CBU β-glucosidase/g glucan | 12.4 | 73.9 | [24] |
Spruce | Steam pretreatment | Tween 20 | In the fermentation stage | SSF | 2 to 10 mm | Washed | 48 | 44 FPU/g cellulose | 20 to 25 | 92 | [15] |
Sugarcane bagasse | Dilute ammonia | Tween 80 | In the pretreatment stage | SSF | 0.05 to 1.5 cm | Washed | 72 | 30 FPU Spezyme CP and 30 CBU Novozyme 188/g glucan | 18 g/100 g of dry biomass | 69 | [23] |
Sugarcane bagasse | Dilute ammonia | Tween 20 | In the pretreatment stage | SSF | 0.05 to 1.5 cm | Washed | 72 | 30 FPU Spezyme CP and 30 CBU Novozyme 188/g glucan | 15 g/100 g of dry biomass | 59 | [23] |