anmoles per μg of total sugar released after 24 hours of hydrolysis from WT (Col-0), pme3, qua2-1, PMEI, and PG plants, quantified using HPAEC-PAD. Values are expressed as nmoles of each sugar per μg of total sugars (TS) in the tissues. Results are means ± SD of three biological replicates. Asterisk (*) indicates statistically significant differences with respect to the WT according to Student’s t-test (P <0.05). The experiments were repeated at least three times with similar results. Ara, arabinose; Col-0, Columbia-0; Gal, galactose; Glc, glucose; HPAEC-PAD, high-performance anion-exchange chromatography with pulsed amperometric detection; PG, polygalacturonase; pme3, pectin methylesterase 3; PMEI, pectin methylesterase inhibitor; qua2-1, quasimodo2-1; WT, wild type; Xyl, xylose.