| | Araa | Gala | Glca | Xyla |
|---|
WT | 0.11 ± 0.05 | 0.24 ± 0.04 | 0.99 ± 0.57 | 0.04 ± 0.02 |
pme3
| 0.11 ± 0.00 | 0.28 ± 0.03 | 2.18 ± 0.32* | 0.05 ± 0.01 |
qua2-1
| 0.14 ± 0.01 | 0.21 ± 0.02 | 1.56. ± 0.05* | 0.03 ± 0.0 |
PMEI | 0.14 ± 0.01 | 0.30 ± 0.06 | 2.22 ± 0.07* | 0.04 ± 0.01 |
PG | 0.13 ± 0.00 | 0.21 ± 0.01 | 1.56 ± 0.09* | 0.05 ± 0.01 |
- anmoles per μg of total sugar released after 24 hours of hydrolysis from WT (Col-0), pme3, qua2-1, PMEI, and PG plants, quantified using HPAEC-PAD. Values are expressed as nmoles of each sugar per μg of total sugars (TS) in the tissues. Results are means ± SD of three biological replicates. Asterisk (*) indicates statistically significant differences with respect to the WT according to Student’s t-test (P <0.05). The experiments were repeated at least three times with similar results. Ara, arabinose; Col-0, Columbia-0; Gal, galactose; Glc, glucose; HPAEC-PAD, high-performance anion-exchange chromatography with pulsed amperometric detection; PG, polygalacturonase; pme3, pectin methylesterase 3; PMEI, pectin methylesterase inhibitor; qua2-1, quasimodo2-1; WT, wild type; Xyl, xylose.
