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Table 1 Analysis of monosaccharides released after enzymatic hydrolysis of leaves from transgenic and mutant plants

From: Analysis of pectin mutants and natural accessions of Arabidopsis highlights the impact of de-methyl-esterified homogalacturonan on tissue saccharification

  Araa Gala Glca Xyla
WT 0.11 ± 0.05 0.24 ± 0.04 0.99 ± 0.57 0.04 ± 0.02
pme3 0.11 ± 0.00 0.28 ± 0.03 2.18 ± 0.32* 0.05 ± 0.01
qua2-1 0.14 ± 0.01 0.21 ± 0.02 1.56. ± 0.05* 0.03 ± 0.0
PMEI 0.14 ± 0.01 0.30 ± 0.06 2.22 ± 0.07* 0.04 ± 0.01
PG 0.13 ± 0.00 0.21 ± 0.01 1.56 ± 0.09* 0.05 ± 0.01
  1. anmoles per μg of total sugar released after 24 hours of hydrolysis from WT (Col-0), pme3, qua2-1, PMEI, and PG plants, quantified using HPAEC-PAD. Values are expressed as nmoles of each sugar per μg of total sugars (TS) in the tissues. Results are means ± SD of three biological replicates. Asterisk (*) indicates statistically significant differences with respect to the WT according to Student’s t-test (P <0.05). The experiments were repeated at least three times with similar results. Ara, arabinose; Col-0, Columbia-0; Gal, galactose; Glc, glucose; HPAEC-PAD, high-performance anion-exchange chromatography with pulsed amperometric detection; PG, polygalacturonase; pme3, pectin methylesterase 3; PMEI, pectin methylesterase inhibitor; qua2-1, quasimodo2-1; WT, wild type; Xyl, xylose.