Figure 3From: Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylasesThe relative activity at (a) different pH and (b) temperature levels, and (c) protein size determination of the recombinant enzymes. (♦) S. cerevisiae Y294[AmyA] and (■) S. cerevisiae Y294[GlaA]. Supernatant of S. cerevisiae Y294[BBH1] (lane 1), S. cerevisiae Y294[AmyA] (lane 2), S. cerevisiae Y294[GlaA] (lane 3), S. cerevisiae Y294[AmyA-GlaA] (lane 4), S. cerevisiae Mnuα1[BBH1] (lane 5), S. cerevisiae Mnuα1[AmyA] (lane 6), S. cerevisiae Mnuα1[GlaA] (lane 7) and S. cerevisiae Mnuα1[AmyA-GlaA] (lane 8) were subjected to SDS-PAGE followed by silver staining. The protein size marker is depicted on the right hand side.Back to article page