Table 1 Microbial strains and plasmids used in this study
From: Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases
Strains or plasmids | Genotype | Source/reference |
|---|---|---|
Strains | Â | Â |
E. coli DH5α | supE44 ΔlacU169 (ϕ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | [26] |
A. tubingensis T8.4 | Wild type | [24] |
S. cerevisiae Y294 | α leu2-3,112 ura3-52 his3 trp1-289 | ATCC 201160 |
S. cerevisiae Y294[BBH1] | URA3 ENO1 P -ENO1 T | This study |
S. cerevisiae Y294[AmyA] | URA3 ENO1 P -amyA-ENO1 T | This study |
S. cerevisiae Y294[GlaA] | URA3 ENO1 P -glaA-ENO1 T | This study |
S. cerevisiae Y294[AmyA-GlaA] | URA3 ENO1 P -amyA-ENO1 T ; URA3 ENO1 P -glaA-ENO1 T | This study |
S. cerevisiae Mnuα1 | haploid ura3, α derivative of MH1000 | [27] |
S. cerevisiae Mnuα1[BBH1] | URA3 ENO1 P -ENO1 T | This study |
S. cerevisiae Mnuα1[AmyA] | URA3 ENO1 P -amyA-ENO1 T | This study |
S. cerevisiae Mnuα1[GlaA] | URA3 ENO1 P -glaA-ENO1 T | This study |
S. cerevisiae Mnuα1[AmyA-GlaA] | URA3 ENO1 P -amyA-ENO1 T ; URA3 ENO1 P -glaA-ENO1 T | This study |
Plasmids | Â | Â |
pTZ57R/T | bla | Fermentas |
pTZ-AmyA | bla amyA | This study |
pTZ-GlaA | bla glaA | This study |
yBBH1 | bla URA3 ENO1 P -ENO1 T | [28] |
yBBH1-AmyA | bla URA3 ENO1 P -amyA-ENO1 T | This study |
yBBH1-GlaA | bla URA3 ENO1 P -glaA-ENO1 T | This study |
yBBH1-AmyA-GlaA | bla URA3 ENO1 P -amyA-ENO1 T ; ENO1 P -glaA-ENO1 T | This study |