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Table 1 Microbial strains and plasmids used in this study

From: Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases

Strains or plasmids Genotype Source/reference
Strains   
E. coli DH5α supE44 ΔlacU16980lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 [26]
A. tubingensis T8.4 Wild type [24]
S. cerevisiae Y294 α leu2-3,112 ura3-52 his3 trp1-289 ATCC 201160
S. cerevisiae Y294[BBH1] URA3 ENO1 P -ENO1 T This study
S. cerevisiae Y294[AmyA] URA3 ENO1 P -amyA-ENO1 T This study
S. cerevisiae Y294[GlaA] URA3 ENO1 P -glaA-ENO1 T This study
S. cerevisiae Y294[AmyA-GlaA] URA3 ENO1 P -amyA-ENO1 T ; URA3 ENO1 P -glaA-ENO1 T This study
S. cerevisiae Mnuα1 haploid ura3, α derivative of MH1000 [27]
S. cerevisiae Mnuα1[BBH1] URA3 ENO1 P -ENO1 T This study
S. cerevisiae Mnuα1[AmyA] URA3 ENO1 P -amyA-ENO1 T This study
S. cerevisiae Mnuα1[GlaA] URA3 ENO1 P -glaA-ENO1 T This study
S. cerevisiae Mnuα1[AmyA-GlaA] URA3 ENO1 P -amyA-ENO1 T ; URA3 ENO1 P -glaA-ENO1 T This study
Plasmids   
pTZ57R/T bla Fermentas
pTZ-AmyA bla amyA This study
pTZ-GlaA bla glaA This study
yBBH1 bla URA3 ENO1 P -ENO1 T [28]
yBBH1-AmyA bla URA3 ENO1 P -amyA-ENO1 T This study
yBBH1-GlaA bla URA3 ENO1 P -glaA-ENO1 T This study
yBBH1-AmyA-GlaA bla URA3 ENO1 P -amyA-ENO1 T ; ENO1 P -glaA-ENO1 T This study
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