Table 3 PCR primers used in the study with the relevant restriction sites in italics (Eco RI = GAATTC; Xho I = CTCGAG)
From: Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases
Primer name | Sequence (5’-3’) | Reference |
|---|---|---|
cDNA amyA | Â | [GenBank: AB008370] |
  AmyA-L | AAGAATTC CGCTTCGCCAAG | |
  AmyA-R | CT GAATTCCTCGAG ATCAACCACCGTC | |
cDNA glaA | Â | [GenBank: D10460] |
  GlaA-L | CAGAATTC CACCGCAATGTCGTTC | |
  GlaA-R | AGCTCGAG AATAGTCTACCGCCAGGT | |
Identification | Â | [41] |
  ITS1 | TCCGTAGGTGAACCTTGCGG | |
  ITS4 | TCCTCCGCTTATTGATATGC |  |