Table 3 PCR primers used in the study with the relevant restriction sites in italics (Eco RI = GAATTC; Xho I = CTCGAG)
From: Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases
| Primer name | Sequence (5’-3’) | Reference |
|---|---|---|
| cDNA amyA | [GenBank: AB008370] | |
| AmyA-L | AAGAATTC CGCTTCGCCAAG | |
| AmyA-R | CT GAATTCCTCGAG ATCAACCACCGTC | |
| cDNA glaA | [GenBank: D10460] | |
| GlaA-L | CAGAATTC CACCGCAATGTCGTTC | |
| GlaA-R | AGCTCGAG AATAGTCTACCGCCAGGT | |
| Identification | [41] | |
| ITS1 | TCCGTAGGTGAACCTTGCGG | |
| ITS4 | TCCTCCGCTTATTGATATGC |