Biotechnology for Biofuels and Bioproducts

Table 1 Sugar consumption and product formation by the best fermenting S. cerevisiae isolates and benchmark strains ^a

From: Exploring grape marc as trove for new thermotolerant and inhibitor-tolerant Saccharomyces cerevisiae strains for second-generation bioethanol production

	S. cerevisiae strains
	27P^b	EC1118^b	MH1000^b	DSM70449^b	F45^c	F56^c	F163^c	Fm17^c
25°C
Glucose, g/l	5.1	7.0	8.1	14.8	3.7	1.0	5.2	4.1
Xylose, g/l	48.2	45.0	46.3	46.7	44.0	46.5	47.9	46.5
Xylitol, g/l	1.7	4.8	3.5	2.9	5.8	3.4	2.0	3.3
Glycerol, g/l	3.6	3.9	3.7	3.6	3.2	3.3	3.1	3.0
Ethanol, g/l	43.8	43.4	40.6	34.9	44.0	46.5	44.9	46.1
Ethanol yield^d	0.46	0.47	0.44	0.41	0.46	0.47	0.47	0.48
Ethanol yield, %^e	90	92	87	80	90	92	93	94
Glycerol yield^f	0.038	0.042	0.040	0.042	0.033	0.033	0.033	0.031
40°C
Glucose, g/l	38.1	43.4	49.2	47.8	16.0	18.5	16.6	15.0
Xylose, g/l	47.4	46.7	48.3	46.3	48.7	48.3	49.4	48.3
Xylitol, g/l	2.2	2.1	2.8	2.8	4.0	3.8	3.8	2.6
Glycerol, g/l	3.0	2.5	2.7	2.8	2.6	2.7	2.8	2.7
Ethanol, g/l	25.5	22.7	18.5	19.5	37.8	38.0	38.1	39.2
Ethanol yield^d	0.41	0.40	0.36	0.37	0.45	0.47	0.46	0.46
Ethanol yield, %^e	81	79	71	73	88	91	90	90
Glycerol yield^f	0.048	0.044	0.053	0.054	0.032	0.033	0.034	0.032

^aMeasured after 21 days fermentation at 25 and 40°C in MNS broth supplemented with glucose (100 g/l) and xylose (50 g/l). All experiments were conducted in triplicate, and relative standard error was always less than 5% (not reported).
^bControl strains.
^cS. cerevisiae isolated from grape marc.
^d,eEthanol yield as ^dgrams of ethanol per gram of consumed glucose and ^epercentage of theoretical maximum (0.51 g/g from glucose).
^fGlycerol yield as grams of glycerol per gram of consumed glucose,

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ISSN: 2731-3654

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