Figure 8
From: Tracking the cellulolytic activity of Clostridium thermocellum biofilms
Correlation between surface footprint area and CO 2 production. A strong positive linear correlation was observed between the measured peak rate of carbon dioxide production and the initial size of Whatman #1 sheets. This shows initial biofilm invasion proceeded to the extent at which available ‘real estate’ was provided, and that surface area was not lost before this point was reached (mainly due to the flat shape of the cellulosic chads; the sheet with the least thickness was chosen for this test). It also emphasizes the utility of such measurements for comparing the degree of bacterium-accessible surface area of complex and heterogeneous substrates. Slope p = 0.000, constant p = 0.560.