Dynamics of oil accumulation and turnover in response to nitrogen status in microplate cultures. (A) Changes in cellular oil content as estimated by the level of Nile red fluorescence. Shaded areas denote the three time points where the corresponding screening was performed. (B) Distribution of Nile red fluorescence at a population level for each time point sampled. AU, arbitrary unit; FL2, fluorescent light channel 2; MM, minimal media; N, nitrogen; TAP, Tris-acetate-phosphate.