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Table 1 A summary of mutants isolated at each step of the screen

From: Development of a forward genetic screen to isolate oil mutants in the green microalga Chlamydomonas reinhardtii

Types of mutants Primary screen (Nile red/flow cytometry) Confirmation step (Nile red/flow cytometry) Validation step (Lipid extraction/TLC quantification)
Type I 8 (0.4%) 8 (0.4%) 5 (0.3%)
Type II 37 (2.1%) 16 (0.9%) 13 (0.7%)
Type III 45 (2.5%) 39 (2.2%) 31 (1.9%)
Total number of mutants; overall rate 76 (4.2%) 41 (2.2%) 31 (1.7%)
Rate of confirmation between the sequential step of screen - 54% 73%
  1. The data x (y%) refers to the number of mutants that are grouped under each type (x), and the rate of their isolation based on a total of 1,800 transformants examined (y%). TLC, thin layer chromatography.
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