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Table 1 A summary of mutants isolated at each step of the screen

From: Development of a forward genetic screen to isolate oil mutants in the green microalga Chlamydomonas reinhardtii

Types of mutants

Primary screen (Nile red/flow cytometry)

Confirmation step (Nile red/flow cytometry)

Validation step (Lipid extraction/TLC quantification)

Type I

8 (0.4%)

8 (0.4%)

5 (0.3%)

Type II

37 (2.1%)

16 (0.9%)

13 (0.7%)

Type III

45 (2.5%)

39 (2.2%)

31 (1.9%)

Total number of mutants; overall rate

76 (4.2%)

41 (2.2%)

31 (1.7%)

Rate of confirmation between the sequential step of screen

-

54%

73%

  1. The data x (y%) refers to the number of mutants that are grouped under each type (x), and the rate of their isolation based on a total of 1,800 transformants examined (y%). TLC, thin layer chromatography.