|
Types of mutants
|
Primary screen (Nile red/flow cytometry)
|
Confirmation step (Nile red/flow cytometry)
|
Validation step (Lipid extraction/TLC quantification)
|
|---|
|
Type I
|
8 (0.4%)
|
8 (0.4%)
|
5 (0.3%)
|
|
Type II
|
37 (2.1%)
|
16 (0.9%)
|
13 (0.7%)
|
|
Type III
|
45 (2.5%)
|
39 (2.2%)
|
31 (1.9%)
|
|
Total number of mutants; overall rate
|
76 (4.2%)
|
41 (2.2%)
|
31 (1.7%)
|
|
Rate of confirmation between the sequential step of screen
|
-
|
54%
|
73%
|
- The data x (y%) refers to the number of mutants that are grouped under each type (x), and the rate of their isolation based on a total of 1,800 transformants examined (y%). TLC, thin layer chromatography.
