Types of mutants | Primary screen (Nile red/flow cytometry) | Confirmation step (Nile red/flow cytometry) | Validation step (Lipid extraction/TLC quantification) |
---|---|---|---|
Type I | 8 (0.4%) | 8 (0.4%) | 5 (0.3%) |
Type II | 37 (2.1%) | 16 (0.9%) | 13 (0.7%) |
Type III | 45 (2.5%) | 39 (2.2%) | 31 (1.9%) |
Total number of mutants; overall rate | 76 (4.2%) | 41 (2.2%) | 31 (1.7%) |
Rate of confirmation between the sequential step of screen | - | 54% | 73% |