Table 5 List of primers used in the study
Gene name | Primer name | Sequences (5’ to 3’) | Usage |
|---|---|---|---|
gfo | GFOR-up-5’ | TGCCGCAGATAAACTCG | Gene disruption |
GFOR-up-kan-3’ | CAGCTCCAGCCTACACAGATTATCTGAAGACGAGA | ||
GFOR-down-kan-5’ | AAGGAGGATATTCATATGTATGAAGCAGCTCGTACC | ||
GFOR-down-3’ | CGCAAGAACCAATACCG | ||
GFOR-int-5’ | AACGGATGACAACGCTT | ||
GFOR-int-3’ | AGTCGTCGTGGTCGAAT | ||
kan | kan-GFOR-5’ | TCTCGTCTTGAGATAATCTGTGTAGGCTGGAGCTG |  |
kan-GFOR-3’ | GGTACGAGCTGCTTCATACATATGAATATCCTCCTT | ||
adhA | adhA-F | CATGAAAGCAGCCGTCA | Real-time PCR |
| Â | adhA-R | TACACCCGCGCAAGTGA | |
adhB | adhB-F | GTCAACGAAATGGGCGA | |
| Â | adhB-R | GTGACGGTCAACAATGG | |
pdc | pdc-F | GACTACAACCTCGTCCT | |
| Â | pdc-R | CAGGGCATGGGAGCAAT | |
16Â s RNA | 16Â s-F | CAGCACCTGTCTCTGATCCA | Â |
| Â | 16Â s-R | GTTCGGAATTACTGGGCGTA | Â |
gfo a | GFOR-com-5’ | GCGAATTC ATGACGAACAAAATCTCG | Complementation |
|  | GFOR-com-3’ | GCGAATTC TCAATAACCACCCTGACG |  |