A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates

Table 1 The set of inter-modular linkers used for cloning

Source organism	Scaffoldin	Module	C-terminal linker	Length (a.a)	Sequence	Accession code
A. cellulolyticus	ScaC	Coh A	Long	29	PTPTQSATPTVTPSATATPTQSATPTVTP	AAP48996
			Short	5	PTPTQ
			None	—	—
B. cellulosolvens	ScaB	Coh B	Long	27	TPTNTISVTPTNNSTPTNNSTPKPNPL	AAT79550
			Short	5	TPTNT
			None	—	—
C. thermocellum	CipA	Coh T	Long	35	PTKGATPTNTATPTKSATATPTRPSVPTNTPTNTP	ABN54273
		Coh T	Short	5	PTKGA
			None	—	—
		CBM (c)	Long	31	VVPSTQPVTTPPATTKPPATTKPPATTIPPS	ABN54273
			Short	5	VVPST
			None	—	—

The preceding module of each linker is indicated. Coh A: the third cohesin of ScaC from A. cellulolyticus, Coh B: the third cohesin of ScaB from B. cellulosolvens, Coh T: The second cohesin of CipA from C. thermocellum, CBM (c): the carbohydrate binding module CBM3a of CipA from C. thermocellum.

ISSN: 2731-3654