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Table 1 The set of inter-modular linkers used for cloning

From: A synthetic biology approach for evaluating the functional contribution of designer cellulosome components to deconstruction of cellulosic substrates

Source organism Scaffoldin Module C-terminal linker Length (a.a) Sequence Accession code
A. cellulolyticus ScaC Coh A Long 29 PTPTQSATPTVTPSATATPTQSATPTVTP AAP48996
Short 5 PTPTQ
None
B. cellulosolvens ScaB Coh B Long 27 TPTNTISVTPTNNSTPTNNSTPKPNPL AAT79550
Short 5 TPTNT
None
C. thermocellum CipA Coh T Long 35 PTKGATPTNTATPTKSATATPTRPSVPTNTPTNTP ABN54273
Short 5 PTKGA
  None
CBM (c) Long 31 VVPSTQPVTTPPATTKPPATTKPPATTIPPS ABN54273
  Short 5 VVPST  
    None  
  1. The preceding module of each linker is indicated. Coh A: the third cohesin of ScaC from A. cellulolyticus, Coh B: the third cohesin of ScaB from B. cellulosolvens, Coh T: The second cohesin of CipA from C. thermocellum, CBM (c): the carbohydrate binding module CBM3a of CipA from C. thermocellum.