Biological saccharification using a C. thermocellum S14 culture supplemented with CglT. Monitoring of cellulose hydrolysis and free sugar (glucose and cellobiose) accumulation during cultivation of C. thermocellum S14 with or without CglT (+CglT and –CglT, respectively). The units are indicated in mM glucose equivalents. Based on an assumed monomer mass of 162 g/mol of microcrystalline cellulose, a 617 mM glucose equivalent was present initially. Red (+CglT) and blue lines (−CglT) indicate residual cellulose content in culture broth. Error bars represent ± SD (n = 3).