Optimization of biological saccharification using C. thermocellum cultures. (A) Effect of CglT activity on biological saccharification. C. thermocellum S14 cultures were supplemented with 10, 30, or 50 U of CglT per g cellulose or left unsupplemented (0 U) at an initial concentration of 100 g/L cellulose. (B) Influence of strain on glucose concentration. C. thermocellum cultures were supplemented with 30 U of CglT per g cellulose at an initial concentration of 100 g/L cellulose. C. thermocellum ATCC 27405 and DSM 1313 were used as type strains for comparison to the originally isolated strain S14. Error bars represent ± SD (n = 3).