High-throughput methodology flow chart. (A) (1) Diverse plant population; (2) harvest the fourth internode; and (3) record in-field geometric measurements. (B) (4) Process into juice and bagasse; (5) dry bagasse; (6) grind dried tissue; and (7) process juice and bagasse. (C) (8) Collect FTIR spectra; (9) PLS prediction of FTIR spectra; and (10) calculate combined total fermentable sugar yield incorporating juice volume (V), sugar concentration ([S]), biomass (B), and digestibility (D) (see Additional file 1 for full protocol). FTIR, Fourier transform infrared; PLS, partial least squares.