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Figure 3 | Biotechnology for Biofuels

Figure 3

From: Assembling a cellulase cocktail and a cellodextrin transporter into a yeast host for CBP ethanol production

Figure 3

Genomic integration of seven gene cassettes in KR7. Each of the seven gene cassettes contains an independent promoter, the alpha factor, a gene coding region, a terminator, and a 55 bp fragment homologous to its neighboring cassette. (A) The gene cassettes assembled in the predesignated order, kanMX, egIII, cbhI, eglA, cbhII, cdtI-gfp, and npabgs, and are shown in red, orange, yellow, green, blue, dark blue, and purple, respectively. (B) The seven gene cassettes were amplified by PCR for transformation with seven primer pairs and the PCR products resulted in seven specific amplicons: 1: kanMX, 2: egIII, 3: cbhI, 4: eglA, 5: cbhII, 6: cdtI-gfp, and 7: npabgs. (C) The order of the gene cassettes was confirmed by PCR with seven internal primer pairs and the PCR products resulted in eight specific amplicons: 1: Lac4-kanMX, 2: kanMX-egIII, 3: egIII-cbhI, 4: cbhI-eglA, 5: eglA-cbhII, 6: cbhII-cdtI-gfp, 7: cdtI-gfp-npabgs, and 8: npabgs- Lac4.

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