Quantitative PCR analysis of the seven gene cassettes in KR7. (A) Relative copy numbers of inserted genes. (B) Relative mRNA levels of cellulase genes inserted in KR7. The relative ratios of the seven genes and their transcripts are shown in comparison to the endogenous alg9 gene in KR7 at 40°C in YPAD culture. The promoter names of the individual genes were given in the brackets.