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Table 1 Activity of LysA, LysA2, LysgaY and λSa2 endolysin against lactic acid bacteria as determined by turbidity (OD 600nm ) reduction analysis

From: Bacteriophage-encoded lytic enzymes control growth of contaminating Lactobacillus found in fuel ethanol fermentations

  Activitya  
Strain LysA LysA2 LysgaY λSa2 Strain Source
Gram positive      
Lactobacillus amylovorus B-4540 - ++ ++ ++ NRRLb
Lactobacillus brevis 0605-48 ++ ++ ++ ++ this study c
Lactobacillus delbrueckii subsp delbrueckii B-763 - - + + NRRL
Lactobacillus fermentum BRO315-1 ++ + + ++ this study
Lactobacillus fermentum BRO315-25 ++ + + +++ this study
Lactobacillus fermentum 0605-B44 +++ + + +++ this study
Lactobacillus fermentum 0713-3 +++ + + +++ this study
Lactobacillus gasseri B-4240 +++ + + +++ NRRL
Lactobacillus hilgardii B-1843 - - - - NRRL
Lactobacillus malefermentans B-1861 - - - ++ NRRL
Lactobacillus paracasei BRO315-44 - - - - this study
Lactobacillus reuteri B-14171 ++ + ++ + NRRL
Staphylococcus aureus Newman + - + +++ Jean C. Leed
Staphylococcus epidermidis + - ++ +++ USDA
Staphylococcus hyicus - - - + USDA
Staphylococcus warneri + - + +++ USDA
Staphylococcus xylocus ++ - ++ +++ USDA
Streptococcus agalactiae ++ - ++ +++ David Pritcharde
Streptococcus dysgalactiae ++ - ++ +++ USDA
Streptococcus pyogenes + - + +++ Dan Nelsonf
Streptococcus suis 531-668 +++ - +++ +++ Dan Nelsonf
Weisella viridescens B-1951 - - + +++ NRRL
Yeast      
Saccharomyces cerevisiae Y-2034 - - - - NRRL
Gram negative      
Escherichia coli DH5α - - - - Invitrogen
  1. a As measured by OD600nm, decrease of whole cell suspensions (initial OD600nm = ~1) treated with lysin (10μM) for 30 min. “+++” between 100% and 75% decrease, “++” between 74% and 50% decrease, “+” less than 49% decrease, “−“no decrease, with respect to no lysin control.
  2. b ARS Culture Collection, Peoria, IL (also known as the NRRL Collection).
  3. c Isolated from fermentors at a commercial dry-grind ethanol facility as described previously [6]. L. fermentum BR0315-1, BR0315-25, and 0713–3 were planktonic isolates, and their MICs for virginiamycin are 16 μg/ml, ≤2 μg/ml, and ≤2 μg/ml, respectively. L. fermentum 0605-B44 was a biofilm isolate from coupon scrapings of a Centers for Disease Control biofilm reactor inoculated with a mash sample from the fermentor.
  4. d Channing Lab, Womens and Brigham Hospital, Boston, MA.
  5. e Dept. Biochemistry, Medical School, Univ. Alabama, Birmingham.
  6. f Department of Veterinary Medicine, UMD, College Park MD.