Expression and subcellular localization of ABC2 and ABC3. (A) Expression of ABC2 and ABC3. The expression of ABC2 and ABC3 carrying 6xHis tag was confirmed by western blot analysis. The positions of molecular mass markers are indicated at left. Western blot analyses were performed as described under “Methods”. (B) Subcellular localization of ABC2 and ABC3 determined by fluorescence microscopy. Yeast cells carrying plasmids encoding ABC2-EGFP or ABC3-EGFP fusion proteins were cultured and harvested. Phase contrast figures and fluorescence figures are shown.