Purification step | Total volume (mL) | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Recovery (%) | Purification (fold) |
---|---|---|---|---|---|---|
Crude extracta | 10 | 2394 | 122 | 20 | 100 | 1 |
Heat treatmentb | 10 | 2155 | 39 | 55 | 90 | 2.8 |
Ni affinity chromatographyc | 1 | 2011 | 17 | 116 | 84 | 5.8 |