Figure 4From: High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel productionBioconversion of tung oil to fatty acid methyl esters by the immobilized r27AROL, free r27AROL, free r28ROL and free mROL. The reaction was carried out by adding 81 U lipase into 10 g tung oil, methanol to oil molar ratio of 3.5:1 (Methanol was added by four steps.) at 4.5 wt.% water content in a 40°C shaker.Back to article page