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Table 1 Independent variables: coded and real value in center composite rotatable design

From: High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel production

Variables Symbols Levels
   −1.68 (−α) −1 0 +1 +1.68(+α)
Initial water added based on oil weight (wt.%) A 0.30 2.00 4.50 7.00 8.70
Methanol to tung oil molar ratio (mol/mol) B 0.98 2.00 3.50 5.00 6.02
Enzyme concentration based on oil weight (wt.%) C 8.3 10.00 12.50 15.00 16.70