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Table 2 Central composite rotatable design arrangement and responses

From: High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel production

  Variable levels Yield (%)
Design point A: water content (wt. %) B: Methanol to oil molar ratio C: Enzyme concentration (wt. %) Experimental Predicted
1 2.00 2.00 10.00 50.7 51.0
2 7.00 2.00 10.00 81.4 79.7
3 2.00 5.00 10.00 70.2 65.8
4 7.00 5.00 10.00 81.2 83.9
5 2.00 2.00 15.00 65.6 62.6
6 7.00 2.00 15.00 77.7 81.8
7 2.00 5.00 15.00 73.4 74.8
8 7.00 5.00 15.00 84.2 83.6
9 0.30 3.50 12.50 46.2 49.4
10 8.70 3.50 12.50 83.7 80.9
11 4.50 0.98 12.50 67.2 67.3
12 4.50 6.02 12.50 80.8 81.2
13 4.50 3.50 8.30 75.8 77.5
14 4.50 3.50 16.70 88.1 86.9
15 4.50 3.50 12.50 92.1 89.5
16 4.50 3.50 12.50 90.9 89.5
17 4.50 3.50 12.50 90.1 89.5
18 4.50 3.50 12.50 90.3 89.5
19 4.50 3.50 12.50 86.1 89.5
20 4.50 3.50 12.50 87.7 89.5