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Table 2 Central composite rotatable design arrangement and responses

From: High-level expression and characterization of a chimeric lipase from Rhizopus oryzae for biodiesel production

 

Variable levels

Yield (%)

Design point

A: water content (wt. %)

B: Methanol to oil molar ratio

C: Enzyme concentration (wt. %)

Experimental

Predicted

1

2.00

2.00

10.00

50.7

51.0

2

7.00

2.00

10.00

81.4

79.7

3

2.00

5.00

10.00

70.2

65.8

4

7.00

5.00

10.00

81.2

83.9

5

2.00

2.00

15.00

65.6

62.6

6

7.00

2.00

15.00

77.7

81.8

7

2.00

5.00

15.00

73.4

74.8

8

7.00

5.00

15.00

84.2

83.6

9

0.30

3.50

12.50

46.2

49.4

10

8.70

3.50

12.50

83.7

80.9

11

4.50

0.98

12.50

67.2

67.3

12

4.50

6.02

12.50

80.8

81.2

13

4.50

3.50

8.30

75.8

77.5

14

4.50

3.50

16.70

88.1

86.9

15

4.50

3.50

12.50

92.1

89.5

16

4.50

3.50

12.50

90.9

89.5

17

4.50

3.50

12.50

90.1

89.5

18

4.50

3.50

12.50

90.3

89.5

19

4.50

3.50

12.50

86.1

89.5

20

4.50

3.50

12.50

87.7

89.5