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Table 1 Cellulases and hemicellulases applied in the hydrolysis experiments

From: Carbohydrate-binding modules (CBMs) revisited: reduced amount of water counterbalances the need for CBMs

Enzymes

  

Intact enzymes

Core enzymes

New name

Old name

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MW

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MW

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μmol/g d.w.

kDa

mg/g d.w.

kDa

mg/g d.w.

TrCel7A

CBH I

0.150

56.0

8.40

47.3

7.09

TrCel6A

CBH II

0.050

56.7

2.84

41.3

2.07

TrCel5A

EG I

0.025

51.9

1.30

42.9

1.07

TrCel7B

EG II

0.025

48.2

1.21

39.6

0.99

TrXyn11

Xylanase II

0.025

20.8

0.52

20.8

0.52

TrMan5A

Mannanase

0.010

47.9

0.48

41.4

0.41

AnCel3A

β-glucosidase

0.002

115.6

0.23

115.6

0.23

Sum

   

14.50 a,b

 

12.00 a,b

  1. a excluding mannanase.
  2. b In the hydrolysis experiments at various substrate consistencies, the total protein loadings equaled 14.5 and 15.0 mg/g d.w. (24.7 and 16.4 mg/g cellulose) intact enzymes and 12.0 and 12.4 mg/g d.w. (20.5 and 13.6 mg/g cellulose) core enzymes in the hydrolysis of wheat straw and Avicel, respectively.
  3. Mannanase was added only to hydrolysis of Avicel.
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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