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Table 1 Cellulases and hemicellulases applied in the hydrolysis experiments

From: Carbohydrate-binding modules (CBMs) revisited: reduced amount of water counterbalances the need for CBMs

Enzymes    Intact enzymes Core enzymes
New name Old name Loading MW Loading MW Loading
   μmol/g d.w. kDa mg/g d.w. kDa mg/g d.w.
TrCel7A CBH I 0.150 56.0 8.40 47.3 7.09
TrCel6A CBH II 0.050 56.7 2.84 41.3 2.07
TrCel5A EG I 0.025 51.9 1.30 42.9 1.07
TrCel7B EG II 0.025 48.2 1.21 39.6 0.99
TrXyn11 Xylanase II 0.025 20.8 0.52 20.8 0.52
TrMan5A Mannanase 0.010 47.9 0.48 41.4 0.41
AnCel3A β-glucosidase 0.002 115.6 0.23 115.6 0.23
Sum     14.50 a,b   12.00 a,b
  1. a excluding mannanase.
  2. b In the hydrolysis experiments at various substrate consistencies, the total protein loadings equaled 14.5 and 15.0 mg/g d.w. (24.7 and 16.4 mg/g cellulose) intact enzymes and 12.0 and 12.4 mg/g d.w. (20.5 and 13.6 mg/g cellulose) core enzymes in the hydrolysis of wheat straw and Avicel, respectively.
  3. Mannanase was added only to hydrolysis of Avicel.