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Table 2 Fermentation products of Caldicellulosiruptor DIB004C alone and in co-cultures with Thermoanaerobacter DIB004G and DIB097X

From: Single-step ethanol production from lignocellulose using novel extremely thermophilic bacteria

Culture Substrate Fermentation products
   Ethanol mM Acetate mM Lactate mM Ethanol:Acetate:Lactate mM:mM:mM Total products mM Ethanol yield mol%
DIB004C Avicel (10 g/l) 2.7 ± 0.2 21.4 ± 0.1 9.0 ± 0.1 1 : 8.0 : 3.4 33.0 ± 0.0 8.1 ± 0.5
DIB004G no growth
DIB097X no growth
DIB004C+DIB004G 8.8 ± 0.7 19.9 ± 0.1 11.0 ± 0.6 1 : 2.3 : 1.3 39.7 ± 1.1 22.1 ± 1.3
DIB004C+DIB097X 10.3 ± 0.7 18.6 ± 0.7 11.8 ± 0.6 1 : 1.8 : 1.1 40.7 ± 0.8 25.4 ± 1.1
DIB004C washed pretreated poplar (2.9 g/l) 1.5 ± 0.1 10.9 ± 0.2 6.2 ± 0.8 1 : 7.2 : 4.1 18.6 ± 0.8 8.1 ± 1.0
DIB004G no growth
DIB097X no growth
DIB004C+DIB004G 5.7 ± 0.3 14.0 ± 0.2 6.6 ± 0.9 1 : 2.5 : 1.2 26.3 ± 1.4 21.7 ± 0.1
DIB004C+DIB097X 3.7 ± 0.2 14.1 ± 0.4 8.3 ± 1.3 1 : 3.9 : 2.3 26.1 ± 1.5 14.0 ± 1.6
DIB004C unwashed pretreated poplar (10 g/l) 2.6 ± 0.2 12.0 ± 0.2 6.1 ± 0.2 1 : 4.6 : 2.3 20.7 ± 0.2 12.6 ± 1.0
DIB004G 4.3 ± 0.3 4.4 ± 0.6 3.0 ± 0.1 1 : 1.0 : 0.7 11.7 ± 0.4 37.0 ± 3.8
DIB097X 5.1 ± 0.1 4.3 ± 0.3 0.0 ± 0.0 1 : 0.8 : 0.0 9.4 ± 0.2 54.5 ± 2.2
DIB004C+DIB004G 5.7 ± 0.4 11.6 ± 0.1 3.1 ± 0.0 1 : 2.0 : 0.5 20.4 ± 0.4 27.9 ± 1.2
DIB004C+DIB097X   7.0 ± 0.1 11.4 ± 0.2 3.5 ± 0.4 1 : 1.6 : 0.5 21.8 ± 0.6 32.1 ± 0.6
  1. Cultures were grown at 72°C for 6 days in flasks with shaking at 100 rpm. Experiments were performed in duplicates.