Strain | Description and characteristics | Reference |
---|---|---|
T. sacch. M1442 | High titer ethanol producing strain | Lee et al., 2011 [47] |
T. sacch. DHC6 | T. sacch ΔxynA::Clo1313_2747 | This study |
T. sacch. DHC15 | T. sacch ΔxynA::cipA* (wild type sec tag) | This study |
T. sacch. DHC16 | T. sacch ΔxynA::cipA* (with a deletion in the sec tag) | This study |
C. therm. DSM 1313 | C. therm wild type obtained from DSMZ culture collection | DSMZ |
C. therm. DS11 | C. therm 1313 ΔcipA | Olson et al. 2013 [48] |
E. coli TOP 10 | Chemically competent | Invitrogen |
S. cerevisiae FY2 | Uracil Auxotroph used for homologous recombination | Winston et. al, 1995 [49] |
Plasmid | Description and characteristics | Reference |
pMC200 | Deletes xynA and replaces it with a kanamycin resistance gene and a removable marker | This study |
pMC212 | Replaces xynA with Clo1313_2747, a kanamycin resistance gene, and a removable marker | This study |
pMC213 | Replaces xynA with cipA*, a kanamycin resistance gene, and a removable marker | This study |
pMC223 | Replaces xynA with Δsec tag cipA*, a kanamycin resistance gene, and a removable marker | This study |
Primer | Sequence | Reference |
xynA_RT_F | TACTTCAGGATGGGTTGGAACAGG | This study |
xynA_RT_R | TCCAATTAGCTGTTCTCCCTGTCG | This study |
gamA_RT_F | ATATTCACCAGCAACGCTGGCTTC | This study |
gamA_RT_R | AATAAGCCTTTGCCAGTTGTCCGC | This study |
Clo1313_2747_RT_F | AGTGGCGTTATACAAACGCTCCTG | This study |
Clo1313_2747_RT_R | ATACAACGGAAGCAACGGCAGAAC | This study |
cipA*_RT_F | ACGACTATCTTTGCCGCTATGATCCC | This study |
cipA*_RT_R | ACTTTAGATCCTACGGCAGCAGTGAC | This study |
pMC200_up_F | gtctttcgactgagcctttcgttttatttgatgcctggatcttttctggcctttaatggcg | This study |
pMC200_up_R | cagctgaagcttcccggggatcctctagagaattcgagctctcttacttcctccctcagtaaatttaatttattg | This study |
pMC200_down_F | ctagataggggtcccgagcgcctacgaggaatttgtatcgaaaaaacaaataatctttaagtaaaaaggcagagagg | This study |
pMC200_down_R | ccgtcagtagctgaacaggagggacagctgatagaagtcaaatgcgacaaaaaaacgcc | This study |