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Table 3 Normalized S/G/H lignin changes

From: Plant cell wall profiling by fast maximum likelihood reconstruction (FMLR) and region-of-interest (ROI) segmentation of solution-state 2D 1H–13C NMR spectra

A: Estimated differences from FMLR

Sample group

S

G

H

 

Δ%

CI

p-value

Δ%

CI

p-value

Δ%

CI

p-value

pal1

1.6

-2.1;5.3

0.73

-1.6

-4.8;1.6

0.57

0.03

-3.5;3.6

1

pal2

0.7

-3.0;4.4

1

-1.4

-4.6;1.8

0.73

0.6

-2.9;4.2

1

c4h

9.2

5.1;13.2

3E-7

-15.9

-19.3;-12.4

1E-12

6.7

2.8;10.6

0.0001

4cl1

10.2

6.5;13.9

1E-9

-13.2

-16.3;-10.0

1E-12

3.0

-0.6;6.5

0.13

4cl2

-1.1

-4.8;2.6

0.94

0.6

-2.6;3.8

1

0.5

-3.1;4.0

1

ccoaomt1

8.2

4.5;11.8

5E-7

-11.6

-14.7;-8.4

1E-12

3.4

-0.1;6.9

0.06

ccr1

-12.5

-16.4;-8.6

6E-12

-2.6

-6.0;0.8

0.18

15.1

11.3;18.9

1E-12

f5h1

-25.0

-28.7;-21.3

1E-12

24.9

21.7;28.1

1E-12

0.1

-3.4;3.7

1

comt

-21.9

-25.6;-18.2

1E-12

21.3

18.2;24.5

1E-12

0.6

-3.0;4.0

1

cad6

0.5

-3.2;4.2

1

-0.2

-3.4;2.9

1

-0.2

-3.8;3.3

1

B: Estimated differences from ROI Integration

Sample group

S

G

H

 

Δ%

CI

p-value

Δ%

CI

p-value

Δ%

CI

p-value

pal1

1.1

-2.2;4.5

0.90

-1.3

-3.9;1.4

0.65

0.1

-2.4;2.7

1

pal2

0.2

-3.1;3.5

1

-0.7

-3.4;2.0

0.98

0.5

-2.1;3.0

1

c4h

7.7

4.0;11.3

2E-6

-12.6

-15.5;-9.6

1E-12

4.9

2.2;7.7

7E-5

4cl1

8.2

4.9;11.5

4E-8

-10.3

-13.0;-7.6

1E-12

2.1

-0.4;4.6

0.14

4cl2

-1.0

-4.4;2.3

0.93

0.6

-2.1;3.3

0.99

0.4

-2.1;3.0

1

ccoaomt1

6.5

3.2;9.8

1E-5

-8.9

-11.6;-6.3

1E-12

2.5

-0.05;5.0

0.06

ccr1

-9.6

-13.1;-6.1

2E-9

-0.5

-3.4;2.3

1

10.1

7.4;12.8

1E-12

f5h1

-21.3

-24.7;-18.0

1E-12

21.3

18.6;23.9

1E-12

0.07

-2.5;2.6

1

comt

-16.7

-20.0;-13.4

1E-12

16.4

13.7;19.1

1E-12

0.3

-2.2;2.9

1

cad6

-0.2

-3.5;3.1

1

0.3

-2.4;3.0

1

-0.1

-2.6;2.4

1

  1. ‘Δ%’ The average predicted differences in the mean percent of S, G, and H lignin between each sample group and the effective wild-type sample group, ‘CI’ the corresponding 95% confidence intervals, ‘p-value’ the Dunnett adjusted p-values. The average predicted differences in Table 3A and Table 3B reflect values calculated from two different ROI quantitative methods: FMLR reconstruction and ROI integration. Values in bold indicate significant differences at an overall significance level of 0.05.