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Table 3 Normalized S/G/H lignin changes

From: Plant cell wall profiling by fast maximum likelihood reconstruction (FMLR) and region-of-interest (ROI) segmentation of solution-state 2D 1H–13C NMR spectra

A: Estimated differences from FMLR
Sample group S G H
  Δ% CI p-value Δ% CI p-value Δ% CI p-value
pal1 1.6 -2.1;5.3 0.73 -1.6 -4.8;1.6 0.57 0.03 -3.5;3.6 1
pal2 0.7 -3.0;4.4 1 -1.4 -4.6;1.8 0.73 0.6 -2.9;4.2 1
c4h 9.2 5.1;13.2 3E-7 -15.9 -19.3;-12.4 1E-12 6.7 2.8;10.6 0.0001
4cl1 10.2 6.5;13.9 1E-9 -13.2 -16.3;-10.0 1E-12 3.0 -0.6;6.5 0.13
4cl2 -1.1 -4.8;2.6 0.94 0.6 -2.6;3.8 1 0.5 -3.1;4.0 1
ccoaomt1 8.2 4.5;11.8 5E-7 -11.6 -14.7;-8.4 1E-12 3.4 -0.1;6.9 0.06
ccr1 -12.5 -16.4;-8.6 6E-12 -2.6 -6.0;0.8 0.18 15.1 11.3;18.9 1E-12
f5h1 -25.0 -28.7;-21.3 1E-12 24.9 21.7;28.1 1E-12 0.1 -3.4;3.7 1
comt -21.9 -25.6;-18.2 1E-12 21.3 18.2;24.5 1E-12 0.6 -3.0;4.0 1
cad6 0.5 -3.2;4.2 1 -0.2 -3.4;2.9 1 -0.2 -3.8;3.3 1
B: Estimated differences from ROI Integration
Sample group S G H
  Δ% CI p-value Δ% CI p-value Δ% CI p-value
pal1 1.1 -2.2;4.5 0.90 -1.3 -3.9;1.4 0.65 0.1 -2.4;2.7 1
pal2 0.2 -3.1;3.5 1 -0.7 -3.4;2.0 0.98 0.5 -2.1;3.0 1
c4h 7.7 4.0;11.3 2E-6 -12.6 -15.5;-9.6 1E-12 4.9 2.2;7.7 7E-5
4cl1 8.2 4.9;11.5 4E-8 -10.3 -13.0;-7.6 1E-12 2.1 -0.4;4.6 0.14
4cl2 -1.0 -4.4;2.3 0.93 0.6 -2.1;3.3 0.99 0.4 -2.1;3.0 1
ccoaomt1 6.5 3.2;9.8 1E-5 -8.9 -11.6;-6.3 1E-12 2.5 -0.05;5.0 0.06
ccr1 -9.6 -13.1;-6.1 2E-9 -0.5 -3.4;2.3 1 10.1 7.4;12.8 1E-12
f5h1 -21.3 -24.7;-18.0 1E-12 21.3 18.6;23.9 1E-12 0.07 -2.5;2.6 1
comt -16.7 -20.0;-13.4 1E-12 16.4 13.7;19.1 1E-12 0.3 -2.2;2.9 1
cad6 -0.2 -3.5;3.1 1 0.3 -2.4;3.0 1 -0.1 -2.6;2.4 1
  1. ‘Δ%’ The average predicted differences in the mean percent of S, G, and H lignin between each sample group and the effective wild-type sample group, ‘CI’ the corresponding 95% confidence intervals, ‘p-value’ the Dunnett adjusted p-values. The average predicted differences in Table 3A and Table 3B reflect values calculated from two different ROI quantitative methods: FMLR reconstruction and ROI integration. Values in bold indicate significant differences at an overall significance level of 0.05.