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Table 2 Lignin content and composition

From: Lignin biosynthesis perturbations affect secondary cell wall composition and saccharification yield in Arabidopsis thaliana

Line

AcBr

H + G + S

% H

% G

% S

% 5-OH-G

%bis-β–O–4-FA

% β–O–4-FA-I

% β–O–4-FA-II

% G aldehydes

% S aldehydes

S/G

WT Col-0

14.5 ± 0.6

817.7 ± 204.7

0.5 ± 0.1

70.3 ± 1.3

29.0 ± 1.4

0.11 ± 0.03

0.07 ± 0.03

0.02 ± 0.008

0.01 ± 0.005

0.02 ± 0.036

0.01 ± 0.006

0.41 ± 0.03

pal1-2

14.0 ± 0.6

793.4 ± 186.8

0.5 ± 0.1

67.0 ± 2.3

32.3 ± 2.3

0.12 ± 0.03

0.06 ± 0.03

0.02 ± 0.004

0.01 ± 0.005

0.01 ± 0.028

0.02 ± 0.009

0.48 ± 0.05

pal1-3

13.9 ± 0.8

812.4 ± 216.4

0.5 ± 0.2

67.7 ± 2.0

31.5 ± 2.0

0.11 ± 0.03

0.05 ± 0.03

0.02 ± 0.007

0.01 ± 0.002

0.02 ± 0.032

0.01 ± 0.005

0.47 ± 0.04

pal2-2

14.1 ± 1.0

821.7 ± 164.3

0.5 ± 0.2

70.3 ± 2.3

29.0 ± 2.2

0.10 ± 0.02

0.07 ± 0.02

0.02 ± 0.006

0.01 ± 0.004

0.02 ± 0.034

0.02 ± 0.008

0.41 ± 0.04

pal2-3

14.2 ± 0.9

818.8 ± 233.5

0.4 ± 0.1

69.2 ± 2.1

30.2 ± 2.0

0.10 ± 0.04

0.05 ± 0.02

0.02 ± 0.009

0.01 ± 0.005

0.03 ± 0.035

0.01 ± 0.011

0.44 ± 0.04

c4h-2

6.0 ± 0.4**

530.7 ± 160.8**

1.9 ± 0.8**

55.7 ± 2.6**

42.1 ± 2.5**

0.15 ± 0.03

0.10 ± 0.07

0.03 ± 0.012

0.02 ± 0.002

0.02 ± 0.020

0.04 ± 0.027

0.76 ± 0.08**

c4h-3

9.3 ± 0.3**

870.7 ± 248.7

0.7 ± 0.2

53.5 ± 2.1**

45.6 ± 2.1**

0.13 ± 0.02

0.08 ± 0.03

0.02 ± 0.008

0.01 ± 0.002

0.01 ± 0.026

0.03 ± 0.026

0.86 ± 0.07**

4cl1-1

10.8 ± 0.5**

828.5 ± 197.4

0.8 ± 0.3

59.9 ± 1.9**

39.1 ± 2.0**

0.12 ± 0.02

0.10 ± 0.04

0.02 ± 0.008

0.01 ± 0.003

0.00 ± 0.002

0.03 ± 0.023

0.65 ± 0.05**

4cl1-2

10.8 ± 0.7**

804.4 ± 151.9

0.8 ± 0.2

60.6 ± 2.7**

38.3 ± 2.1**

0.11 ± 0.05

0.10 ± 0.04

0.02 ± 0.010

0.01 ± 0.004

0.02 ± 0.055

0.02 ± 0.014

0.63 ± 0.06**

4cl2-1

14.3 ± 0.7

783.3 ± 169.1

0.5 ± 0.2

69.6 ± 2.3

29.6 ± 2.3

0.12 ± 0.02

0.07 ± 0.03

0.02 ± 0.007

0.01 ± 0.003

0.02 ± 0.040

0.01 ± 0.004

0.43 ± 0.05

4cl2-3

14.0 ± 0.5

819.7 ± 179.8

0.5 ± 0.1

70.9 ± 1.7

28.3 ±1.8

0.12 ± 0.03

0.08 ± 0.03

0.02 ± 0.006

0.01 ± 0.004

0.00 ± 0.004

0.01 ± 0.009

0.40 ± 0.04

ccoaomt1-3

11.4 ± 1.7**

789.3 ± 156.8

0.6 ± 0.1

62.3 ± 4.4**

36.7 ± 4.3**

0.13 ± 0.03

0.14 ± 0.06

0.04 ± 0.010

0.02 ± 0.008

0.02 ± 0.029

0.01 ± 0.011

0.60 ± 0.11**

ccoaomt1-5

10.9 ± 0.4**

696.1 ± 187.9

0.9 ± 0.3

60.7 ± 2.9**

38.0 ± 3.0**

0.12 ± 0.03

0.14 ± 0.03

0.08 ± 0.113

0.03 ± 0.023

0.03 ± 0.036

0.02 ± 0.007

0.63 ± 0.08**

ccr1-3

7.1 ± 0.7**

192.1 ± 49.3**

4.5 ± 1.4**

71.7 ± 4.9

18.5 ± 5.0**

0.23 ± 0.11

2.83 ± 1.91**

1.38 ± 0.569**

0.81 ± 0.218**

0.04 ± 0.030

0.09 ± 0.048**

0.26 ± 0.09**

ccr1-6

6.9 ± 0.4**

229.3 ± 79.0**

1.2 ± 1.2*

68.7 ± 2.6

24.9 ± 2.7**

0.17 ± 0.08

2.55 ± 1.04**

1.61 ± 0.366**

0.94 ± 0.317**

0.05 ± 0.061

0.06 ± 0.042

0.36 ± 0.05

f5h1-2

15.9 ± 0.6*

596.0 ± 167.4**

0.4 ± 0.1

99.3 ± 0.2**

0.0 ± 0.0**

0.03 ± 0.01

0.10 ± 0.05

0.04 ± 0.013

0.02 ± 0.008

0.02 ± 0.025

0.01 ± 0.009

0.00 ± 0.00**

f5h1-4

15.3 ± 0.1

595.6 ± 137.0**

0.4 ± 0.1

99.3 ± 0.2**

0.0 ± 0.0**

0.03 ± 0.01

0.08 ± 0.04

0.04 ± 0.012

0.03 ± 0.007

0.02 ± 0.031

0.01 ± 0.003

0.00 ± 0.00**

comt-1

13.7 ± 1.0

499.6 ± 151.0**

0.7 ± 0.2

97.7 ± 0.6**

0.5 ± 0.1**

0.93 ± 0.32**

0.09 ± 0.04

0.03 ± 0.012

0.02 ± 0.009

0.05 ± 0.070

0.02 ± 0.025

0.01 ± 0.00**

comt-4

14.1 ± 0.9

502.8 ± 177.2**

0.7 ± 0.3

94.9 ± 0.6**

3.2 ± 0.4**

0.94 ± 0.35**

0.10 ± 0.02

0.04 ± 0.016

0.02 ± 0.018

0.05 ± 0.079

0.01 ± 0.007

0.03 ± 0.00**

cad6-1

14.5 ± 0.8

779.7 ± 183.7

0.3 ± 0.1

73.7 ± 2.1*

25.5 ± 2.1*

0.10 ± 0.02

0.10 ± 0.04

0.02 ± 0.006

0.01 ± 0.004

0.04 ± 0.061

0.16 ± 0.132**

0.35 ± 0.04

cad6-4

14.4 ± 0.7

742.0 ± 206.3

0.3 ± 0.1

75.3 ± 4.2**

23.9 ± 4.3**

0.16 ± 0.14

0.09 ± 0.02

0.03 ± 0.008

0.01 ± 0.005

0.02 ± 0.030

0.18 ± 0.122**

0.32 ± 0.07**

  1. Lignin content and composition for the 20 lignin mutants (n = 8) and wild-type (WT) (n = 16) (± SD). Lignin content was determined with the AcBr assay and expressed as percentage CWR. Lignin composition was determined with thioacidolysis. The sum of H, G, and S is expressed in μmol g-1 AcBr lignin. The relative proportions of the different lignin units were calculated based on the total thioacidolysis yield (including the minor nonconventional lignin units). S/G was calculated based on the absolute values for S and G (expressed in μmol g-1 AcBr lignin). bis-β–O–4-FA: G-CHR-CHR2; β–O–4-FA-I: G-CH = CH-COOH; β–O–4-FA-II: G-CHR-CH2-COOH. Increases and decreases compared to the wild-type are in bold and italics, respectively. * 0.001 < p < 0.01; ** p < 0.001 (Dunnett-Hsu adjusted t-test).