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Table 2 Lignin content and composition

From: Lignin biosynthesis perturbations affect secondary cell wall composition and saccharification yield in Arabidopsis thaliana

Line AcBr H + G + S % H % G % S % 5-OH-G %bis-β–O–4-FA % β–O–4-FA-I % β–O–4-FA-II % G aldehydes % S aldehydes S/G
WT Col-0 14.5 ± 0.6 817.7 ± 204.7 0.5 ± 0.1 70.3 ± 1.3 29.0 ± 1.4 0.11 ± 0.03 0.07 ± 0.03 0.02 ± 0.008 0.01 ± 0.005 0.02 ± 0.036 0.01 ± 0.006 0.41 ± 0.03
pal1-2 14.0 ± 0.6 793.4 ± 186.8 0.5 ± 0.1 67.0 ± 2.3 32.3 ± 2.3 0.12 ± 0.03 0.06 ± 0.03 0.02 ± 0.004 0.01 ± 0.005 0.01 ± 0.028 0.02 ± 0.009 0.48 ± 0.05
pal1-3 13.9 ± 0.8 812.4 ± 216.4 0.5 ± 0.2 67.7 ± 2.0 31.5 ± 2.0 0.11 ± 0.03 0.05 ± 0.03 0.02 ± 0.007 0.01 ± 0.002 0.02 ± 0.032 0.01 ± 0.005 0.47 ± 0.04
pal2-2 14.1 ± 1.0 821.7 ± 164.3 0.5 ± 0.2 70.3 ± 2.3 29.0 ± 2.2 0.10 ± 0.02 0.07 ± 0.02 0.02 ± 0.006 0.01 ± 0.004 0.02 ± 0.034 0.02 ± 0.008 0.41 ± 0.04
pal2-3 14.2 ± 0.9 818.8 ± 233.5 0.4 ± 0.1 69.2 ± 2.1 30.2 ± 2.0 0.10 ± 0.04 0.05 ± 0.02 0.02 ± 0.009 0.01 ± 0.005 0.03 ± 0.035 0.01 ± 0.011 0.44 ± 0.04
c4h-2 6.0 ± 0.4** 530.7 ± 160.8** 1.9 ± 0.8** 55.7 ± 2.6** 42.1 ± 2.5** 0.15 ± 0.03 0.10 ± 0.07 0.03 ± 0.012 0.02 ± 0.002 0.02 ± 0.020 0.04 ± 0.027 0.76 ± 0.08**
c4h-3 9.3 ± 0.3** 870.7 ± 248.7 0.7 ± 0.2 53.5 ± 2.1** 45.6 ± 2.1** 0.13 ± 0.02 0.08 ± 0.03 0.02 ± 0.008 0.01 ± 0.002 0.01 ± 0.026 0.03 ± 0.026 0.86 ± 0.07**
4cl1-1 10.8 ± 0.5** 828.5 ± 197.4 0.8 ± 0.3 59.9 ± 1.9** 39.1 ± 2.0** 0.12 ± 0.02 0.10 ± 0.04 0.02 ± 0.008 0.01 ± 0.003 0.00 ± 0.002 0.03 ± 0.023 0.65 ± 0.05**
4cl1-2 10.8 ± 0.7** 804.4 ± 151.9 0.8 ± 0.2 60.6 ± 2.7** 38.3 ± 2.1** 0.11 ± 0.05 0.10 ± 0.04 0.02 ± 0.010 0.01 ± 0.004 0.02 ± 0.055 0.02 ± 0.014 0.63 ± 0.06**
4cl2-1 14.3 ± 0.7 783.3 ± 169.1 0.5 ± 0.2 69.6 ± 2.3 29.6 ± 2.3 0.12 ± 0.02 0.07 ± 0.03 0.02 ± 0.007 0.01 ± 0.003 0.02 ± 0.040 0.01 ± 0.004 0.43 ± 0.05
4cl2-3 14.0 ± 0.5 819.7 ± 179.8 0.5 ± 0.1 70.9 ± 1.7 28.3 ±1.8 0.12 ± 0.03 0.08 ± 0.03 0.02 ± 0.006 0.01 ± 0.004 0.00 ± 0.004 0.01 ± 0.009 0.40 ± 0.04
ccoaomt1-3 11.4 ± 1.7** 789.3 ± 156.8 0.6 ± 0.1 62.3 ± 4.4** 36.7 ± 4.3** 0.13 ± 0.03 0.14 ± 0.06 0.04 ± 0.010 0.02 ± 0.008 0.02 ± 0.029 0.01 ± 0.011 0.60 ± 0.11**
ccoaomt1-5 10.9 ± 0.4** 696.1 ± 187.9 0.9 ± 0.3 60.7 ± 2.9** 38.0 ± 3.0** 0.12 ± 0.03 0.14 ± 0.03 0.08 ± 0.113 0.03 ± 0.023 0.03 ± 0.036 0.02 ± 0.007 0.63 ± 0.08**
ccr1-3 7.1 ± 0.7** 192.1 ± 49.3** 4.5 ± 1.4** 71.7 ± 4.9 18.5 ± 5.0** 0.23 ± 0.11 2.83 ± 1.91** 1.38 ± 0.569** 0.81 ± 0.218** 0.04 ± 0.030 0.09 ± 0.048** 0.26 ± 0.09**
ccr1-6 6.9 ± 0.4** 229.3 ± 79.0** 1.2 ± 1.2* 68.7 ± 2.6 24.9 ± 2.7** 0.17 ± 0.08 2.55 ± 1.04** 1.61 ± 0.366** 0.94 ± 0.317** 0.05 ± 0.061 0.06 ± 0.042 0.36 ± 0.05
f5h1-2 15.9 ± 0.6* 596.0 ± 167.4** 0.4 ± 0.1 99.3 ± 0.2** 0.0 ± 0.0** 0.03 ± 0.01 0.10 ± 0.05 0.04 ± 0.013 0.02 ± 0.008 0.02 ± 0.025 0.01 ± 0.009 0.00 ± 0.00**
f5h1-4 15.3 ± 0.1 595.6 ± 137.0** 0.4 ± 0.1 99.3 ± 0.2** 0.0 ± 0.0** 0.03 ± 0.01 0.08 ± 0.04 0.04 ± 0.012 0.03 ± 0.007 0.02 ± 0.031 0.01 ± 0.003 0.00 ± 0.00**
comt-1 13.7 ± 1.0 499.6 ± 151.0** 0.7 ± 0.2 97.7 ± 0.6** 0.5 ± 0.1** 0.93 ± 0.32** 0.09 ± 0.04 0.03 ± 0.012 0.02 ± 0.009 0.05 ± 0.070 0.02 ± 0.025 0.01 ± 0.00**
comt-4 14.1 ± 0.9 502.8 ± 177.2** 0.7 ± 0.3 94.9 ± 0.6** 3.2 ± 0.4** 0.94 ± 0.35** 0.10 ± 0.02 0.04 ± 0.016 0.02 ± 0.018 0.05 ± 0.079 0.01 ± 0.007 0.03 ± 0.00**
cad6-1 14.5 ± 0.8 779.7 ± 183.7 0.3 ± 0.1 73.7 ± 2.1* 25.5 ± 2.1* 0.10 ± 0.02 0.10 ± 0.04 0.02 ± 0.006 0.01 ± 0.004 0.04 ± 0.061 0.16 ± 0.132** 0.35 ± 0.04
cad6-4 14.4 ± 0.7 742.0 ± 206.3 0.3 ± 0.1 75.3 ± 4.2** 23.9 ± 4.3** 0.16 ± 0.14 0.09 ± 0.02 0.03 ± 0.008 0.01 ± 0.005 0.02 ± 0.030 0.18 ± 0.122** 0.32 ± 0.07**
  1. Lignin content and composition for the 20 lignin mutants (n = 8) and wild-type (WT) (n = 16) (± SD). Lignin content was determined with the AcBr assay and expressed as percentage CWR. Lignin composition was determined with thioacidolysis. The sum of H, G, and S is expressed in μmol g-1 AcBr lignin. The relative proportions of the different lignin units were calculated based on the total thioacidolysis yield (including the minor nonconventional lignin units). S/G was calculated based on the absolute values for S and G (expressed in μmol g-1 AcBr lignin). bis-β–O–4-FA: G-CHR-CHR2; β–O–4-FA-I: G-CH = CH-COOH; β–O–4-FA-II: G-CHR-CH2-COOH. Increases and decreases compared to the wild-type are in bold and italics, respectively. * 0.001 < p < 0.01; ** p < 0.001 (Dunnett-Hsu adjusted t-test).