Novel monosaccharide fermentation products in Caldicellulosiruptor saccharolyticus identified using NMR spectroscopy

Table 2 Major fermentation products in the batch culture supernatants as a function of sugar substrate

Monosaccharide	Acetate^a	Lactate^a	Ethanol	Ethylene glycol	1,2-propanediol^b	Glycerol^a	Acetoin^c	2,3-butanediol^b	Hydroxyacetone^b
D-glucose (Glu)	37.2	1.4	4.1	0.0	0.0	3.5	trace	0.4	trace
D-mannose (Man)	25.3	11.3	2.3	0.0^d	0.2	2.2	0.1	0.0	1.7
D-arabinose (D-Ara)	32.1	2.2	1.8	23.0^a	0.1	3.0	0.0^d	0.0	0.0
L-arabinose (L-Ara)	27.0	12.3	6.1	0.0	0.1	1.7	0.1	0.0	3.8
D-xylose (Xyl)	34.6	6.4	2.1	0.0^d	0.0	3.3	0.1	0.0	3.5
D-fucose (D-Fuc)	36.6	11.0	3.8	0.9	2.2	9.1	0.0	0.0	0.0
L-fucose (L-Fuc)	33.5	8.0	6.7	0.3	ND^e	7.1	0.0	0.0	trace

The unit of the metabolites is mM/OD_600.
^a The concentrations of major metabolites were determined using HPLC.
^b Estimated concentrations were based on manual spectral peak deconvolution, rather than by automated (Chenomx) method.
^c Acetoin could be quantified by the Chenomx 7.61 software, not the Chenomx 7.51 version.
^d The metabolites could be detected in the continuous cultures, not in the batch cultures.
^e Concentration could not be accurately determined.

ISSN: 2731-3654