Figure 3

An example of screening for n -butanol tolerance of a single deletion strain in the CEN.PK background, comparing IMK356 ( pre9 Δ) (●) and CEN.PK113-7D (□). The strains were grown in 96 well plates in synthetic medium containing different concentrations of n-butanol, and the OD₆₆₀ was measured after 48 h. Each point represents the average final OD₆₆₀ and standard deviation for each n-butanol concentration, calculated from at least 16 independent cultures.