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Table 2 Single nucleotide variation identified in the evolved strains IMS0344 and IMS0351 by whole-genome resequencing

From: Genome-scale analyses of butanol tolerance in Saccharomyces cerevisiae reveal an essential role of protein degradation

Strain

Mutated allele

Nucleotide change

Amino acid change

IMS0344

rpn4-1

G1518C

K506N

 

rtg1-1

A235G

K79E

 

ssk2-1

C3974A

P1325Q

 

ubr1-1

A3194G

E1065G

IMS0351

rpn4-2

C1546T

Q516*

 

rtg1-2

C256A

L86I

 

nma111-2

C545A

S182*

 

ubr1-2

C2129A

S710*

 

sto1-2

T2543G

F736V

 

rpl10-2

change from G to C 296 bp upstream of RPL10,

——————

 

stt4-2

change from T to C 984 bp upstream of SST4.

——————

  1. * denotes the introduction of an early stop codon.